The invention provides a composite
fluorescence coding method for a biomarker and application of the composite
fluorescence coding method. A composite fluorescent coding method for a biomarker comprises the following steps: a
composite structure formed by connecting tetrahedral
DNA frames with the same side length in series is used as a coding carrier, one of the tetrahedral
DNA frames is used for loading fluorescent coding dyes with different colors to realize the distinguishing of different biological targets, and the other tetrahedral
DNA frame is used for loading fluorescent coding dyes with different colors; the other tetrahedral DNA framework is used for loading quantitative dye and a recognition probe, so that quantitative analysis of the
biological target is realized. Single chains respectively extend out of the same sides of the tetrahedral DNA framework, and are connected in series and assembled according to a base complementary
pairing principle to obtain the coding vector. According to the invention, a
composite structure formed by series connection of TDF is used as the coding vector, so that the problems of biotoxicity, easy degradation by
nuclease and the like of a traditional inorganic material and a two-dimensional DNA nano-material are solved, and long-term
structural stability of the coding vector in living cells is realized.