The invention provides a lithium ion battery isolation film, which includes a porous substrate and a polymer coating coated on at least one side surface of the porous substrate; and the material of the polymer includes at least one of polyacrylic acid, polymethacrylic acid, polymethyl acrylate, styrene copolymer, neopentyl glycol diacrylate, polytetrafluoroethylene, polyimide, polyamide, polyester, polysulfone and polyacrylonitrile. The polymer has good bonding performance, the prepared polymer coating prepared by the invention is thin in thickness, uniform and continuous in distribution, andthe adhesion among the porous substrate, the inorganic coating and the positive and negative electrode sheets can be improved, thereby improving the hardness of the battery cell. In addition, the polymer particles have a large number of micro-pores between them, and the polymer particles swelling in the electrolyte is small, which can ensure that the obtained isolation film has good air permeability and battery cyclicity.