The application discloses a construction method of a road and bridge safety guardrail, and comprises the following steps: (a) marking a construction area; (b) installing a first cross beam between two stand columns to obtain a stand column combination; (c) installing a buffer cylinder between two stand column combinations; (d) butting a second cross beam with a connecting seat, and then installing the connecting seat on the first cross beam; (e) butting the second cross beam with two adjacent connecting seats; (f) fixing the stand column in place; (g) installing on the second cross beam; (h) locking a first anti-glare plate; and (i) installing a limiting rod between two adjacent anti-glare plates. The application has the advantages of simple construction, small quantity of bolts, low operation difficulty, low labor intensity, high construction efficiency, step-by-step order, guaranteed
assembly quality of the guardrail, reliable and effective light blocking wall formed by the anti-glare plates, difficult penetration of light of an opposite vehicle, good light blocking effect, and solved problem of opposite vehicle light glare.