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Embryogenic culture initiation of douglas-fir by maltose

A technology of maltose and Douglas fir, which is applied in tissue culture, microorganism, plant regeneration, etc., and can solve problems such as stimulation

Inactive Publication Date: 2007-09-19
WEYERHAEUSER CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] An ongoing problem with somatic cell cloning of Douglas-fir embryos is the stimulation of efficient initiation and establishment of Douglas-fir embryogenic cells capable of generating somatic embryos

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example shows a comparison of the probability of initiation and formation of Douglas-fir stalk mass obtained using initiation and maintenance media containing either maltose or sucrose as the main sugar source.

[0044] Cone cells were collected from four hybrid mother trees (All Congeny) when embryos were at the pre-round cell and round cell stages of development. Pyramidal cells were transferred on blue ice and stored in plastic bags in a cold room at 0-4°C until dissection was required. Deseed and dissect the pyramidal cells on the same day. After removing the seeds, place into a beaker containing pre-sterilized water.

[0045] Divide the seeds into equal portions of 60 to 80 seeds. Each serving is placed in a separate pre-sterilized tea strainer before subsequent procedures. First, the seeds were agitated for 10 minutes in 10% Liqui-nox (2 drops of whole Tween-20 per liter). After rinsing the seeds in deionized water (18 megohm) for 30 min, the tea strainer...

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PUM

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Abstract

In one aspect, the present invention provides methods for producing Douglas-fir somatic embryos. In one aspect, the methods of the invention comprise the step of culturing Douglas-fir explants in, or on, an initiation medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form embryonal suspensor masses comprising early-stage somatic embryos. In another aspect, the methods of the invention additionally comprise the step of culturing Douglas-fir embryonal suspensor masses in maintenance medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form an established embryonal suspensor mass comprising early-stage somatic embryos.

Description

field of invention [0001] The present invention relates to a method for producing Douglas fir somatic embryos. Background of the invention [0002] The demand for manufactured wood products from coniferous trees such as pine and fir continues to increase. A proposed solution to this problem is to identify individual trees with desirable characteristics such as rapid growth, and generate large numbers of genetically identical clones of superior trees by somatic cell cloning. [0003] Somatic cloning is the process of producing a genetically identical tree from tree tissue rather than male and female gametes. In one method of somatic cell cloning, plant tissue is cultured in a starting medium that includes hormones such as auxins and / or cytokines to initiate embryogenic cells such as the stalk mass, the Embryogenic cells are capable of developing to form somatic embryos. The embryogenic cells are further cultured in a maintenance medium to facilitate the establishment and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00A01H7/00C12NC12N5/02
CPCA01H4/005
Inventor 巴莫德·K·古普塔戴安娜·G·霍尔姆斯特伦多丽丝·布德沃思
Owner WEYERHAEUSER CO