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Cell culturation apparatus

A cell culture and seed cell technology, which is applied to tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc. Simple structure and cost reduction effect

Inactive Publication Date: 2008-03-26
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] At the same time, in the current methods of inducing stem cells to differentiate into specific cell types (such as nerve cells, muscle cells, insulin-secreting cells, osteoblasts, fat cells, etc.), most of them add exogenous chemical reagents and cytokine combinations to create stem cells. In the microenvironment of directed differentiation, the cost of culture is still high, and at the same time, various factor reagents appear at different time points with the consumption of time, there are concentration gradients, and the induction system is not stable enough.

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Embodiment Construction

[0015] Please match Figure 1, Figure 2, and Figure 3, which is a cell culture device provided by the present invention, which mainly includes:

[0016] A cell culture tank 1, one end of which is provided with a seed cell inlet 11, a nutrient solution inlet 12, and a stromal cell addition port 5, and its other end is provided with a cultured cell outlet 13, a metabolic waste liquid outlet 14, and a stromal cell discharge port. Outlet 6; the cell culture tank 1 is provided with at least two nutrient solution supply pipes 15, each nutrient solution supply pipe 15 is a blind pipe, one end close to the metabolic waste liquid outlet 14 is closed, and the other end is connected to the nutrient solution inlet 12 ; The cell culture tank 1 is provided with a metabolic waste liquid recovery tube 16, which is also a blind tube, one end close to the nutrient solution inlet 12 is closed, and the other end is connected to the metabolic waste liquid outlet 14; At least two cell culture tubes ...

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Abstract

This invention discloses a cell cultivatioin device including: a cell cultivation tank with an inlet for seed cells, an inlet for nutrient fluid and a stromal cell adding port on one end, the other end is set with an outlet for cultivated cells, an outlet for metabolized waste solution and a discharge port for stromal cells, at least two supplying tubes for nutrient fluid, one metabolized waste solution reclaim tube and two cell cultivation tubes are set in the cell cultivation tank, other places of which are fully filled with stromal cells, a nutrient fluid circulation device connected with the inlet of the nutrient fluid and the wasted outlet of the cell cultivation tank, a cell providing device connected with the seed cell inlet of the tank and a cell collecting device connected with the outlet of the cultivated cells of the tank.

Description

technical field [0001] The invention relates to a cell culture device, in particular to a device capable of rapidly culturing in large quantities in vitro to obtain specific cells, especially stem cells and their daughter cells of different degrees of differentiation. Background technique [0002] Stem cells are considered ideal seed cells for cell therapy and tissue engineering because of their self-renewal and multi-directional differentiation potential. Both cell therapy and tissue engineering require a considerable number of stem cells. However, the source of stem cells is limited. The current planar culture operations such as ordinary multi-well plates and square flasks are complicated. The intermittent changes in the growth microenvironment have a great impact on cell growth. The number of harvested cells It is far from reaching the clinical needs. At the same time, directly adding a combination of cytokines to the culture system will greatly increase the cost of cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
Inventor 裴雪涛张鹏习佳飞王韫芳闫舫吉蕾刘雨潇
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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