Unlock instant, AI-driven research and patent intelligence for your innovation.

Neisseria meningitidis antigens and compositions

A composition and antibody technology, applied in the direction of drug combinations, antibodies, microorganisms, etc., can solve the problems of incompleteness and inability to overcome antigenic variation.

Inactive Publication Date: 2008-04-09
CHIRON CORP +1
View PDF85 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other proteins used in outer membrane vaccines are the opa and opc proteins, but none of these approaches can overcome antigenic variation (for example, Ala' Aldeen and Borriello (1996) "meningococcal transferrin-binding proteins 1 and 2 are exposed, And produce bactericidal antibodies capable of killing homologous and heterologous strains ("Vaccine 14(1):49-53)
[0009] A certain amount of sequence information is available for genes and proteins of meningococci and gonorrhoeae (eg EP-A-0467714, WO96 / 29412), but this is by no means complete

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Neisseria meningitidis antigens and compositions
  • Neisseria meningitidis antigens and compositions
  • Neisseria meningitidis antigens and compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0390] Using the procedure described above, the following oligonucleotide primers were employed in a polymerase chain reaction (PCR) assay to clone the indicated ORFs:

[0391] Table 1 is used for the oligonucleotide in the PCR of embodiment 2-10

[0392]

[0393] Positioning of ORFs

[0394]The following DNA and amino acid sequences are qualified with headings of the form: [g, m, or a][#].[seq or pep], where "g" indicates that the sequence is from Neisseria gonorrhoeae and "m" indicates that the sequence is from meninges Neisseria B, and "a" indicates that the sequence is from N. meningitidis A; "#" indicates the sequence number; "seq" indicates the DNA sequence, and "pep" indicates the amino acid sequence. For example, "g001.seq" refers to the N. gonorrhoeae DNA sequence numbered 1. When the suffix "-1" is present for these sequences, it indicates additional sequences found for the same ORF, therefore, the ORF data for sequence nomenclature without and with suffix apply...

Embodiment 2

[0637] Expression of ORF 919

[0638] The primers described in Table 1 for ORF 919 were used to map and clone ORF 919. The predicted gene 919 was cloned in pET vector and expressed in E. coli. Protein expression products and purified products were analyzed by SDS-PAGE. In panel A) the analysis of the purification of the 919-His fusion protein is shown. Mice were immunized with purified 919-His, and serum was used for Western blot analysis (Panel B), FACS (Panel C), bactericidal test (Panel D) and ELISA analysis (Panel E). Symbols: M1, molecular weight marker; PP, purified protein; TP, N. meningitidis total protein extract; OMV, N. meningitidis outer membrane vesicle preparation. Arrows indicate the location of the major recombinant protein product (A) and N. meningitidis immunogen-reactive bands (B). These experiments confirmed that 919 is a surface-exposed protein and a useful immunogen. Hydrophobicity map, antigenic index, and amphipathic region of ORF 919 in Figure...

Embodiment 3

[0640] Expression of ORF 279

[0641]The primers for ORF 279 described in Table 1 were used to map and clone ORF 279. The predicted gene 279 was cloned in pGex vector and expressed in E. coli. Protein expression products and purified products were analyzed by SDS-PAGE. In panel A) the analysis of the purification of the 279-GST fusion protein is shown. Mice were immunized with purified 279-GST, and serum was used for Western blot analysis (Panel B), FACS (Panel C), bactericidal test (Panel D) and ELISA analysis (Panel E). Symbols: M1, molecular weight marker; TP, N. meningitidis total protein extract; OMV, N. meningitidis outer membrane vesicle (vesicle) preparation. Arrows indicate the location of the major recombinant protein product (A) and N. meningitidis immunogen-reactive bands (B). These experiments confirmed that 279 is a surface-exposed protein and a useful immunogen. Hydrophobicity map, antigenic index, and amphipathic region of ORF 279 in Figure 11 availabl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides proteins from Neisseria gonorrhoeae and Neisseria meningitidis, including the amino acid sequences and the corresponding nucleotide sequences. The proteins are predicted to be useful antigens for vaccines and / or diagnostics.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of April 30, 1999, an application number of CN1373806A, and an invention title of "Neisseria meningitidis antigen and composition". [0002] This application is a continuation-in-part of the following U.S. Provisional Patent Applications, each of which is hereby incorporated by reference in its entirety: 60 / 083,758 (filed May 1, 1998), 60 / 094,869 (filed July 31, 1998), 60 / 098,994 (filed September 2, 1998), 60 / 099,062 (filed September 2, 1998), 60 / 103,749 (filed October 9, 1998) , 60 / 103,794 (filed 9 October 1998), 60 / 103,796 (filed 9 October 1998), and 60 / 121,528 (filed 25 February 1999). technical field [0003] The present invention relates to antigens of the following bacterial species: Neisseria meningitidis and Neisseria gonorrhoeae. Background technique [0004] Neisseria meningitidis is an immobile, gram-negative diplococcus pathogenic to humans. Neisseria m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/22C07K16/12C12N15/31A61K39/395A61P31/04C12Q1/68G01N33/68G01N33/569G01N33/50A61K38/00A61K39/00A61K39/095C12N15/09C12P21/08C12R1/36
CPCY10S530/806Y10S530/825A61K38/00C07K14/22A61K39/00A61P31/04A61P37/02A61P37/04A61K39/095A61K2039/55505
Inventor C·弗雷泽C·加莱奥蒂G·格兰迪E·希基V·马斯格阿尼M·莫拉J·彼得森M·皮扎R·拉普奥利G·拉蒂E·斯卡拉托M·斯卡塞利H·特特林J·C·文特尔
Owner CHIRON CORP