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A segment differential nucleotide sequence of smallpox virus genom

A nucleotide sequence, smallpox virus technology, applied in antiviral agents, recombinant DNA technology, microbe measurement/testing, etc., can solve the problems of the latter without substantial progress, unable to distinguish smallpox virus from other poxviruses, etc.

Inactive Publication Date: 2008-04-16
中国疾病预防控制中心病毒病预防控制所
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the former still cannot distinguish smallpox virus from other poxviruses, and the latter has not yet made substantial progress

Method used

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  • A segment differential nucleotide sequence of smallpox virus genom
  • A segment differential nucleotide sequence of smallpox virus genom
  • A segment differential nucleotide sequence of smallpox virus genom

Examples

Experimental program
Comparison scheme
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example 1

[0017] Example 1. Searching for the specific sequence of the variola virus genome by comparing the variola virus genome with other virus genomes

[0018] Use Pairwise BLAST software to compare the homology of the variola virus genome sequence and other viruses (see attached table 1 for details) one by one. The results showed that there was only one region in the variola virus genome that was specific to all other virus genomes that participated in the comparison, and it was named the variola virus specific sequence U160K (results are shown in Figure 1). The sequence is 920 nucleotides long and contains two protein coding frames, located at the 5'-end of the sequence from the 207th nucleotide to the 500th nucleotide and from the 516th nucleotide to the 713rd core Nucleic acid (see accompanying drawing 2).

example 2

[0019] Example 2. Using FASTA software to further confirm the specificity of the variola virus genome-specific sequence U160K

[0020] Using FASTA software, the variola virus specific sequence U160K was compared with all published viral nucleotide sequences (a total of 154,696) recorded in the EMBL database (up to October 2002). The results showed that, except for the nucleotide sequence of variola virus, no virus was significantly homologous to the U160K sequence (results are shown in Figure 3), thereby confirming the specificity of the sequence.

example 3

[0021] Example 3. The sequence U160K exists and is conserved in smallpox virus

[0022] The sequence U160K was compared with the genomes of three variola virus genomes, namely, the common variola virus Indian strain and the Bengal strain, and the light variola virus Garcia strain. The results show (see Fig. 4 for details): the sequence is homologous to the 160681st nucleotide to the 161600th nucleotide region of the 5'-end of the Indian strain genome record strand and is arranged continuously, and the homology ratio is 100%; The region from the 160713th nucleotide to the 161680th nucleotide of the 5'-end of the genome record chain of the strain is homologous and continuously arranged, with a homology ratio of 94.0%; The nucleotides to the 163261st nucleotide region are homologous but arranged intermittently, with a homology ratio of 94.7%.

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Abstract

The present invention belongs to the fields of biotechnology and virus detecting technology. The present invention provides one section of variola virus genome specific nucleotide sequence U160K, which has length of 920 bases and exists widely in variola virus genome. The nucleotide sequence has different distribution modes in common type variola virus and light variola virus, has nucleotide homology greater than 90 %, and has no obvious homology to virus other than variola virus. It is the genetic matter mark specific to variola virus and may be used in the detection technology research and development of variola virus specificity.

Description

technical field [0001] The present invention generally relates to biotechnology, in particular to the detection of smallpox virus and smallpox diagnostic technology. Background technique [0002] Smallpox is a severe infectious disease caused by the smallpox virus belonging to the genus Orthopoxvirus of the family Poxviridae. It can be transmitted through the respiratory tract and skin and mucous membranes among people of all ages. Historically, there was a large-scale epidemic, and the case fatality rate was as high as 20%-30%. Due to the widespread vaccination of vaccinia (smallpox vaccine), the epidemic of smallpox was effectively prevented, and smallpox was finally eradicated worldwide in the 1970s. Since 1980, vaccinia vaccination has been stopped all over the world. At present, there are a large number of people who have no immunity or low immunity to smallpox virus in the population. There are at least 400 million people under the age of 22 in my country who are not ...

Claims

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Application Information

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IPC IPC(8): C07H21/00C12N15/11C12Q1/68A61K31/7088A61P31/20
Inventor 阮力王世峰郑楠陆柔剑
Owner 中国疾病预防控制中心病毒病预防控制所
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