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Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer

A technology for compounding microorganisms and fertilizers, which is applied in the field of strains and their compositions, and can solve problems that have not been reported

Inactive Publication Date: 2009-06-24
新疆惠森生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no products at home and abroad that microbial fertilizers can comprehensively achieve nitrogen fixation, phosphorus and potassium solutions, and there are no technical reports.

Method used

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  • Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer
  • Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer
  • Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1: XHS0026N strain and its function in microbial fertilizer

[0103] The microbial strain XHS0026N was identified as Klebsiella pneumoniae subsp pneumonaiae CGMCC.No.1642 after microbiological identification.

[0104] 1. The medium formula of this strain, including:

[0105] 1.1. Slant solid medium: 10 grams of sugar, K 2 HPO 4 0.5 g, CaCO 3 1 g, MgSO 4 0.2g, 0.2g NaCl, 1000ml water, 18g agar.

[0106] 2.2. Shaking flask liquid culture medium: sugar 5g-7g, KH 2 PO 4 0.7 g, K 2 HPO 4 0.3 g, MgSO 4 ·7H 2 O 0.5 g, CaCO 3 1 g, NaCl 0.5 g, FeCl 3 0.01 g, Na 2 MoO 4 ·2H 2 O 0.01g, yeast juice 0.9g, Na 3 C 6 H 5 O 7 ·2H 2 0.01 g of O and 1000 ml of water.

[0107] 2. Ingredients for seed pot: 700 grams of white sugar, KH 2 PO 4 50 grams, K 2 HPO 4 30 grams, MgSO 4 ·7H 2 O 50 grams, CaCO 3 100g, NaCl 50g, FeCl 3 1 g, Na 2 MoO 4 ·H 2 O 1 g, yeast juice 100 g, Na 3 C 6 H 5 O 7 ·H 2 O 1 g.

[0108] 3. Fermentation tank ingredients:

[0109] Loading factor: 0.7, volume after in...

Embodiment 2

[0123] Example 2: XHS0012K strain and its function in microbial fertilizer

[0124] The XHS0012K strain in the microbial fertilizer was identified as Bacillus mucilaginosus CGMCC.No.1641 through microbiological identification.

[0125] 1. The medium formula of this strain, including

[0126] 1.1. Preparation method of the production slope: (used for the cultivation and preservation of the production slope bacteria)

[0127] Proportion: (g) sucrose 2-12, K 2 HPO 4 0.1-0.8, MgSO 4 0.05-0.4, Nac10.03-0.6, CaCO 3 0.4-1.2, CaSO 4 0.03-0.3, KH 2 PO 4 0.05-0.3, yeast juice 0.1-0.7, sodium citrate 0.02-0.2, agar 20, distilled water 1000ml.

[0128] Culture conditions: 30°C, 2-4 days. The surface of the culture medium is rich in exosporous polysaccharides, and the cells are normal during microscopic examination, and there are a small amount of spores. It can be stored in a refrigerator at minus 5°C and taken as needed. The shelf life is no more than one month. If the cotton plug of the obl...

Embodiment 3

[0151] Example 3: XHS0019P strain and its function in microbial fertilizer

[0152] The microorganism XHS0019P strain was identified as Bacillus megaterium CGMCC. No. 1640 by microbiological identification.

[0153] 1. The medium formula of this strain, including

[0154] 1.1. Beef extract, peptone, yeast extract culture medium: peptone 2-7g, beef extract 0.8-6g, yeast extract 1-5g, NaCl 3-8g, glucose agar 15-20g, distilled water 1000ml, adjust pH7 .0, adding 2-10mg MnSo4·H 2o is beneficial to spore production.

[0155] 1.2. Potato culture medium: 500ml potato extract, 15-20g sucrose or glucose, 20g agar, 500ml distilled water.

[0156] Peel 1600-2000 grams of potatoes, wrap them in cloth, put them in 4500ml of water, and boil for 10-30 minutes. Cultivation conditions: 30°C, culture for 2-3 days.

[0157] 2. Krishna bottle production method

[0158] 2.1. Medium: peptone 2-7g, beef extract 0.8-6g, yeast extract 1-5g, NaCl 3-8g, glucose agar 15-20g, distilled water 1000ml, adjust pH...

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Abstract

The present invention discloses a composite microbial fertilizer. It is characterized by utilizing three bacterial powders of Bacillus mucilaginosus CGMCC, No.1641, Klebsiella pneumoniae subsp pneumonaiae CGMCC, No.1642 and Bacillus megaterium CGMCC, No.1640, mixing them according to weight ratio of 1:1:1 to obtain their mixture and mixing said mixture with a synergistic agent according to a certain mixing ratio so as to obtain the invented microbial fertilizer.

Description

Technical field: [0001] The invention relates to a strain and its composition in the field of microbial fertilizers, and specifically refers to a mixture of three strains having the functions of fixing nitrogen, dissolving phosphorus, and dissolving potassium in the field of microbial fertilizer. . Background technique: [0002] Internationally, microbial fertilizers use a wide range of bacteria, including various rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-releasing bacteria, photosynthetic bacteria, etc. In recent years, there have been more and more bacterial species with PGPR effect. The EM bacteria and enzyme bacteria invented in Japan respectively contain 5 families, 10 genera, 80 species and 3 genera and 24 species of bacteria, actinomycetes, yeasts, etc. It can be seen that there are many types of bacteria used in the production of microbial fertilizers in the world. [0003] The earliest microbial fertilizer (inoculant) product in the w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07C12R1/11C12R1/22
Inventor 李秀荣魏亚新王心歌陈明韩友庆
Owner 新疆惠森生物技术有限公司
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