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Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer

A technology of compound microorganisms and compositions, applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as unreported

Inactive Publication Date: 2007-01-31
新疆惠森生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no products at home and abroad that microbial fertilizers can comprehensively achieve nitrogen fixation, phosphorus and potassium solutions, and there are no technical reports.

Method used

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  • Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer
  • Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer
  • Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1: XHS0026N strain and its function in microbial fertilizer

[0102] Microbial strain XHS0026N was determined to be Klebsiella pneumoniae subsp pneumoniae subspecies (klebsiella pneumoniae subsp pneumoniaiae CGMCC.No.1642) through microbiological identification.

[0103] 1. The culture medium formula of the strain, including:

[0104] 1.1. Inclined solid medium: 10 grams of sugar, K 2 HPO 4 0.5 g, CaCO 3 1 g, MgSO 4 0.2 grams, 0.2 grams of NaCl, 1000 ml of water, and 18 grams of agar.

[0105] 2.2. Shake flask liquid medium: sugar 5g-7g, KH 2 PO 4 0.7 grams, K 2 HPO 4 0.3 g, MgSO 4 ·7H 2 O 0.5 g, CaCO 3 1 g, NaCl 0.5 g, FeCl 3 0.01 g, Na 2 MoO 4 2H 2 O 0.01g, yeast juice 0.9g, Na 3 C 6 h 5 o 7 2H 2 O 0.01 grams, water 1000ml.

[0106] 2. Ingredients for the seed tank: 700 grams of sugar, KH 2 PO 4 50 grams, K 2 HPO 4 30 grams, MgSO 4 ·7H 2 O 50g, CaCO 3 100g, NaCl 50g, FeCl 3 1 g, Na 2 MoO 4 ·H 2 O 1g, yeast juice 100g, ...

Embodiment 2

[0122] Example 2: XHS0012K strain and its function in microbial fertilizer

[0123] The XHS0012K strain in the microbial fertilizer was determined to be Bacillus mucilaginosus CGMCC.No.1641 through microbiological identification.

[0124] 1. The culture medium formula of the strain, including

[0125] 1.1. Production method of slant: (for the cultivation and preservation of slant strains)

[0126] Ratio: (grams) sucrose 2-12, K 2 HPO 4 0.1-0.8, MgSO 4 0.05-0.4, Nacl 0.03-0.6, CaCO 3 0.4-1.2, CaSO 4 0.03-0.3, KH 2 PO 4 0.05-0.3, yeast juice 0.1-0.7, sodium citrate 0.02-0.2, agar 20, distilled water 1000ml.

[0127] Culture conditions: 30°C, 2-4 days. The surface of the culture medium is rich in exospore polysaccharides. Microscopically, the bacteria are normal and there are a small amount of spores. They can be stored in a refrigerator at 5°C above zero and taken out at any time. The storage period is not more than one month. If the cotton plug of the inclined te...

Embodiment 3

[0150] Embodiment 3: XHS0019P bacterial strain and its function in microbial fertilizer

[0151] Microbial strain XHS0019P was identified as Bacillus megaterium (Bacillus megaterium CGMCC.No.1640) through microbiological identification.

[0152] 1. The culture medium formula of the strain, including

[0153] 1.1. Beef extract, peptone, yeast extract medium: peptone 2-7g, beef extract 0.8-6g, yeast extract 1-5g, NaCl 3-8g, glucose agar 15-20g, distilled water 1000ml, adjust pH7 .0, adding 2-10mg MnSo4·H2o is beneficial to produce spores.

[0154] 1.2. Potato medium: 500ml of potato extract, 15-20g of sucrose or glucose, 20g of agar, 500ml of distilled water.

[0155] Peel 1600-2000 grams of potatoes, wrap them in cloth, put them in 4500ml of water, and boil for 10-30 minutes. Culture conditions: 30°C, culture for 2-3 days.

[0156] 2. Kirschner bottle method

[0157] 2.1. Medium: 2-7 grams of peptone, 0.8-6 grams of beef extract, 1-5 grams of yeast extract, 3-8 grams of Na...

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PUM

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Abstract

The present invention discloses a composite microbial fertilizer. It is characterized by utilizing three bacterial powders of Bacillus mucilaginosus CGMCC, No.1641, Klebsiella pneumoniae subsp pneumonaiae CGMCC, No.1642 and Bacillus megaterium CGMCC, No.1640, mixing them according to weight ratio of 1:1:1 to obtain their mixture and mixing said mixture with a synergistic agent according to a certain mixing ratio so as to obtain the invented microbial fertilizer.

Description

Technical field: [0001] The present invention relates to a strain and its compound in the field of microbial fertilizer, specifically refers to the mixture obtained by compounding three strains with functions of nitrogen fixation, phosphorus dissolution and potassium dissolution in microbial fertilizer and special synergist . Background technique: [0002] There are a wide range of strains used in microbial fertilizers in the world, including various rhizobia, nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, and photosynthetic bacteria. In recent years, more and more strains with PGPR effects have been reported. The EM bacteria and enzyme bacteria invented in Japan respectively contain 5 families, 10 genera, 80 species and 3 genera with 24 species of bacteria, actinomycetes, yeasts, etc. It can be seen that there are many types of bacteria used in the production of microbial fertilizers in the world. [0003] The earliest microbi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07C12R1/10C12R1/22
Inventor 李秀荣魏亚新王心歌陈明韩友庆
Owner 新疆惠森生物技术有限公司
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