Thrombolytic medicine synergist and its preparing process

A technology for thrombolytic drugs and synergists, applied in the field of biopharmaceuticals, can solve the problems of patent applications and literature reports that no synergists are found, and achieve the effects of improving thrombolytic effects, reducing usage, and reducing drug costs.

Inactive Publication Date: 2007-08-15
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no patent applications and literature reports about P11 as a synergist of thrombolytic drugs have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thrombolytic medicine synergist and its preparing process
  • Thrombolytic medicine synergist and its preparing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Cloning of human P11 protein gene and construction, screening and identification of prokaryotic expression plasmid

[0039] Strains and expression vectors: the expression strains Escherichia coli BL21, E.coli DH5α, and plasmid pBV220 are all preserved by the Institute of Basic Medical Sciences, Academy of Military Medical Sciences of the Chinese People's Liberation Army. They are all publicly available elsewhere.

[0040] Enzymes and biochemical reagents: restriction enzymes BamHI, EcoR I, Taq DNA polymerase and T 4 DNA ligase was purchased from TaKaRa Company; nucleic acid molecular weight standard DL2000 was purchased from TaKaRa Company; protein molecular weight standard was purchased from Jingke Bioengineering Company; DNA purification kit was from Boda Biotechnology Company; plasmid extraction kit was from Promega Company. The Bradford method protein quantification kit was purchased from Pleibo Company. Human thrombin, human plasminogen, human fibrinogen, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a synergist for thrombolysis and its preparation method, which primarily adopts widely available P11protein as synergist for thrombolysis and other medicine. The synergist can enhance the activity of drugs for thrombolysis, improve thrombolysis effect thereby reducing medicamentous consumption and relieve adverse side effect, and reducing cost.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a thrombolytic drug synergist and a preparation method. Background technique [0002] P11 protein (calpactinI, light chain) is a member of the S100 family (Schafer BW et al., Trends Biochem Sci, 1996, 21(4): 134-140), also known as S100A10, is a polypeptide containing 97 amino acid residues (11Kda). P1 1 protein has an EF-hand type structure and exists in the form of helix-loop-helix (S. Rety, et al., Nat. Struct. Biol. 6 (1999) 89-95.27). P11 protein usually regulates the blood fibrinolytic system (G.Kassam, B etc., Biochemistry37 (1998) 16958-16966.) as the subunit of annexin (annexinII) heterotetramer complex (AIIt), can be alone or with membrane Annexin II stimulates tissue-type plasminogen activity factor (t-PA) in the form of a complex to regulate plasminogen activity (Travis J. et al., Biological Chemistry, 2003, 278 (28): 25577-25584 ). In the human body,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61P7/02C12P21/02C12N15/12A61K38/17
Inventor 徐东刚姚广印邹民吉王旻房涛
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap