Verdigris pseudomonads testing fluid and simple testing bottle

A Pseudomonas aeruginosa, test bottle technology, applied in the direction of microbial determination/inspection, biochemical instruments, biochemical equipment and methods, etc., can solve the problem of lengthy time, extremely high test environment and operator quality requirements, and wrong test results To solve the problem of judging and other problems, to achieve the effect of saving infrastructure investment, accurate and reliable test results, and avoiding contamination and interference

Inactive Publication Date: 2010-10-06
白莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current traditional determination method of Pseudomonas aeruginosa is to use the combination of enrichment isolation culture and a variety of biochemical tests to judge. Bacteria→④Medium preparation→⑤Dilution and inoculation of water samples→⑥Enrichment culture→⑦Isolation culture→⑧Gram staining microscope inspection→⑨Biochemical test→⑩result report, it usually takes 10 days to complete the whole test process. Traditional methods not only operate The procedure is cumbersome, complicated, and time-consuming, and it has extremely high requirements on the test environment and the quality of the operators. The whole monitoring process and Control procedures have strict regulations and requirements
In addition, the complicated test process can easily produce large errors, resulting in misjudgment of test results

Method used

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  • Verdigris pseudomonads testing fluid and simple testing bottle
  • Verdigris pseudomonads testing fluid and simple testing bottle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment one: see figure 1 In the figure, 1 is an aluminum skin cover, 2 is a rubber stopper, 3 is a glass bottle chamber with equal cross-section, and 4 is a test solution for Pseudomonas aeruginosa. h 1 is the height of Pseudomonas aeruginosa test solution, H 2 Empty height for the equal section of the test bottle. In the present embodiment, the test bottle is a colorless transparent oral liquid bottle of 15mL, and the height H of the test solution is 1 is 6cm, and the height of the space is H 2 It is 4cm.

[0021] The formula of the above test solution is: 0.02% cetyltrimethylammonium bromide, 0.2% sodium chloride, 0.5% peptone, 0.1% beef extract. In a colorless transparent oral liquid bottle, it can be sterilized by high pressure for 15 minutes.

[0022] When in use, the extinction dilution method is used, that is, the water sample to be determined is injected into the test bottle step by step with a sterile syringe (solid samples must be dissolved or extrac...

Embodiment 2

[0023] Embodiment two: the present embodiment is basically the same as embodiment one, and the difference is that the test bottle is a colorless transparent vial of 8mL, and the height H of the test solution is 1 is 4.5cm, and the height of the space is H 2 It is 3.5cm.

[0024] The formula of the above test solution is: 0.15% cetyltrimethylammonium bromide, 1.0% chloride, 2.0% peptone, 0.5% beef extract, and the balance is distilled water. After the above-mentioned test solutions are prepared, pack 4.5 mL per bottle into 8 mL colorless transparent vials, and sterilize under high pressure for 15 minutes.

[0025] When using, use a sterile syringe to draw 0.5mL water quality sample, inject it into the test bottle, shake it well and place it in a 37°C drying oven or water bath for 24 hours, and judge the negative or positive reaction according to the change of the liquid in the bottle, which is qualitative. Analysis; make 2 or 3 sets of parallel samples according to this metho...

Embodiment 3

[0026] Embodiment three: the present embodiment is basically the same as embodiment one, and the difference is that the test bottle is a colorless transparent round-bottomed glass bottle of 10mL, and the height H of the test solution is 1 is 3.0cm, and the height of the space is H 2 It is 0.9cm.

[0027] The formula of the above test solution is: cetyltrimethylammonium bromide 0.04, sodium chloride 0.2, peptone 0.5%, beef extract 0.1%, distilled water 96.4-99.6%, and the balance is distilled water. After the above test solution is prepared, pack 4.5 ml of each bottle in a 10 mL test bottle and autoclave for 15 minutes.

[0028] Using method is the same as embodiment one.

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Abstract

A method for testing microbe, its test liquid and tester are disclosed. The blue verditer pseudomonads test liquid contains cetyl trimethyl ammonium bromide 0.02-0.15%, chloride 0.2-1.0%, proteintone 0.5-2.0%, beef educt 0.1-0.5% and distilled water 96.35-99.18%. Height H1 of test liquid: constant-section space height H2=1:0.3-0.8. It's fast, convenient, correct and has long storage time. It can be used for water-quality, food, medicines and cosmetics.

Description

technical field [0001] The invention relates to a method for testing microorganisms in water, food and other substances and a testing device thereof, in particular to a test liquid for Pseudomonas aeruginosa in water or other substances and a test bottle thereof. Background technique [0002] Pseudomonas aeruginosa (Pseudomonas aeruginosa) is widely distributed in nature. For humans, it is an opportunistic pathogen. Long-term use of hormones, immunosuppressants, tumor chemotherapy, radiation therapy, etc. lead to low immune function of patients, and post-operative Or patients after certain treatment operations (tracheotomy, retention of urinary catheter, etc.) are likely to cause infection with this bacterium, so it is considered that this bacterium is one of the important pathogenic bacteria for nosocomial infection. Two research reports recently published in the "New England Journal of Medicine" show that the problem of Pseudomonas aeruginosa water pollution has attracted ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12M1/34
Inventor 宋毛平李树华刘五一白莉陈恭恩
Owner 白莉
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