Method for fast breeding western azalea and culture medium therefor
A test tube flowering and culture medium technology, applied in horticultural methods, botany equipment and methods, applications, etc., can solve the problems of small yield, difficulty in meeting the needs of the market, and long cultivation period of reproduction technology, etc., to achieve short cultivation period, split good branch growth
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[0083] Embodiments of the invention. Adopt the rapid propagation method that tender stem tip and stem section form cluster bud, carry out flower bud induction to its test-tube plantlet on the basis of rapid propagation, it comprises the following steps:
[0084] a. Preparation of explants
[0085] Take the 1-1.5 cm long stem tip and stem segment of the newly-sent young stem containing 1-3 axillary bud nodes, put it under tap water for 1-2 hours, and gently stir it 2-3 times with a glass rod during this period, so that Wash off the dust and bacteria on its surface; rinse with 70% alcohol for 30-60 seconds under sterile conditions, rinse once with sterile water, and transfer to 0.1% HgCl 2 (Mercuric chloride) solution was sterilized for 6-8 minutes, rinsed with sterile water 4-5 times; remove the blackened part of the stem tip and stem segment, and put it in a sterile petri dish equipped with filter paper for later use.
[0086] b. Establishment and proliferation of sterile cu...
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