Method for fast breeding western azalea and culture medium therefor

A test tube flowering and culture medium technology, applied in horticultural methods, botany equipment and methods, applications, etc., can solve the problems of small yield, difficulty in meeting the needs of the market, and long cultivation period of reproduction technology, etc., to achieve short cultivation period, split good branch growth

Inactive Publication Date: 2007-10-03
GUIZHOU ACAD OF SCI
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  • Application Information

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Problems solved by technology

In recent years, with the improvement of people's living standards, the demand is increasing. The traditional propagation technology has a long cultivation cy...

Method used

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Embodiment Construction

[0083] Embodiments of the invention. Adopt the rapid propagation method that tender stem tip and stem section form cluster bud, carry out flower bud induction to its test-tube plantlet on the basis of rapid propagation, it comprises the following steps:

[0084] a. Preparation of explants

[0085] Take the 1-1.5 cm long stem tip and stem segment of the newly-sent young stem containing 1-3 axillary bud nodes, put it under tap water for 1-2 hours, and gently stir it 2-3 times with a glass rod during this period, so that Wash off the dust and bacteria on its surface; rinse with 70% alcohol for 30-60 seconds under sterile conditions, rinse once with sterile water, and transfer to 0.1% HgCl 2 (Mercuric chloride) solution was sterilized for 6-8 minutes, rinsed with sterile water 4-5 times; remove the blackened part of the stem tip and stem segment, and put it in a sterile petri dish equipped with filter paper for later use.

[0086] b. Establishment and proliferation of sterile cu...

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Abstract

A tissue culture method for the fast reproduction of Western azalea and making its test-tube plantlet to bloom includes such steps as preparing the explant, creating an aseptic culture system, reproduction, culturing the strong test-tube plantlet, rooting culture, and inducing the test-tube plantlet to bloom. Its culture medium is also disclosed.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation and test-tube flowering method of Rhododendron azalea and the nutritional components used, belonging to the technical field of flower cultivation. Background technique [0002] Western Rhododendron (commonly known as Western Cuckoo) (Rhododendron hybridum west) was listed as one of the world's top ten potted flowers in the early days, and it is very popular in the international flower market. Its flowering period is as long as forty or fifty days, and it is more resistant to viewing than ordinary rhododendrons. In addition to being used for ornamental potted plants, it can also be used for many purposes such as garden decoration and forming flower hedges. In recent years, with the improvement of people's living standards, the demand is increasing. The traditional propagation technology has a long cultivation cycle and low output, and is also affected by seasons and cutting propagation mother ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H3/00C12N5/04
Inventor 陈训刘燕巫华美
Owner GUIZHOU ACAD OF SCI
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