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Probiotics freeze drying process

A technology of probiotics and freeze-drying, applied in the direction of bacteria, microorganisms, fungi, etc., can solve problems such as difficult to master and complicated steps

Inactive Publication Date: 2007-10-03
邵峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the freeze-drying process occupies an extremely important position in the preservation of microorganisms, but due to strict requirements, certain equipment is required, and the steps are complicated and difficult to master

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preservation method of probiotics is realized through the following steps:

[0018] The yeast was inserted into TY (Yeast Tryptone Agar) medium according to the inoculum amount of 8%. Streptococcus faecalis was inserted into the MRS medium at an inoculum of 8%, and Lactobacillus was inserted into the MRS medium at an inoculum of 8%.

[0019] Ferment the inoculated strains in the fermenter, the fermentation temperature is 37°C; the inner pressure of the tank is 0.3 `kg / cm2, the yeast is aerobic fermentation, and the fecal streptococcus and lactobacillus are sterile air fermentation.

[0020] The fermented preparation is centrifuged under the condition of 2000fpm in a centrifuge to obtain the sludge,

[0021] Add 3.5% skimmed milk powder and 1.0% glucose to the obtained bacteria slime according to weight percentage, respectively, to mix and embed bacteria.

[0022] The acquired bacterial strains were respectively placed in a freeze dryer to make freeze-dried powder. ...

Embodiment 2

[0025] A preservation method of probiotics is realized through the following steps:

[0026] The yeast was inserted into TY (Yeast Tryptone Agar) medium according to the inoculum amount of 10%. Streptococcus faecalis was inoculated into MRS medium at 10% inoculation amount, and Lactobacillus was inoculated into MRS medium at 10% inoculum amount.

[0027] Ferment the inoculated strains in the fermentation tank, the fermentation temperature is 37°C; the pressure inside the tank is 0.6 `kg / cm2, the yeast is aerobic fermentation, and the fecal streptococcus and lactobacillus are sterile air fermentation,

[0028] The fermented preparation is centrifuged under the condition of 2500fpm in a centrifuge to obtain the sludge,

[0029] Add 4.0% skimmed milk powder and 1.5% glucose to the obtained bacteria slime according to the weight percentage, respectively, to mix and embed the bacteria.

[0030] The acquired bacterial strains were respectively placed in a freeze dryer to make fre...

Embodiment 3

[0033] A preservation method of probiotics is realized through the following steps:

[0034] The yeast was inserted into TY (Yeast Tryptone Agar) medium according to the inoculum amount of 9%. Streptococcus faecalis was inserted into the MRS medium at a 9% inoculation amount, and Lactobacillus was inserted into the MRS medium at a 9% inoculum amount.

[0035] Ferment the inoculated strains in the fermenter respectively, the fermentation temperature is 36°C; the internal pressure of the tank is 0.5 `kg / cm2, the yeast is aerobic fermentation, and the fecal streptococcus and lactobacillus are sterile air fermentation,

[0036] The fermented preparation is centrifuged under the condition of 2500fpm in a centrifuge to obtain the sludge,

[0037] Add 4.0% skimmed milk powder and 1.5% glucose to the obtained bacteria slime according to the weight percentage, respectively, to mix and embed the bacteria.

[0038] The acquired bacterial strains were respectively placed in a freeze dr...

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Abstract

The present invention relates to microbe protecting technology, and is especially probiotics freeze drying process. The technological process includes inoculating yeast, faeces and lactobacillus in the amount of 8-10 % each, fermenting inside a tank at 37-39 deg.c temperature and 0.3-0.6 Kg / sq cm pressure, centrifuging the fermented product at 2000-2500 rpm to obtain bacterial mud, adding protecting agent to embed spawn, and freeze drying in a freeze drying machine to obtain freeze dried powder. The process is suitable for protection in short term and simple.

Description

Technical field: [0001] The invention relates to a microorganism freeze-drying technology, in particular to a freeze-drying technology of probiotics. Background technique: [0002] Microorganisms play an extremely important role in nature. The life activity of microorganisms has a short generation period, and they are prone to mutation or even death during the passage process, which may cause the loss of excellent strains. Therefore, strain preservation is the process of microbial breeding. An important part of the preservation of strains, the preservation of strains often reduces the growth and metabolic activity of the strains by artificially creating conditions to reduce the variation. Generally, by keeping the nutrient content of the medium at the lowest level, anoxic state, dryness and low temperature, the strains are in "dormant state". Among them, the freeze-drying process occupies an extremely important position in the preservation of microorganisms, but due to stri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/14C12R1/225C12R1/46C12R1/85
Inventor 邵峰温建新
Owner 邵峰
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