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Red cell G 6PD deficiency disease double enzyme ratio detection method and its reagent kit and usage method

A technology for deficiency and red blood cells, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long process flow and only one-by-one detection, and achieve the effects of improving detection efficiency, reducing waste liquid volume, and reducing costs

Inactive Publication Date: 2007-10-24
杜传书 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the manual double-enzyme ratio method has a long process flow, and the samples can only be tested one by one. The maximum limit of a day can only detect 20-30 samples, and the reaction volume of each sample is 2060μl×2.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] One of embodiment 1 kit composition

[0019] Component: Glucose-6-phosphate sodium reaction solution (R2 / G6P)

[0020] It consists of 1 part (parts by volume, the same below) of 500uMol glucose-6-phosphate sodium (G6P), 3 parts of 0.2mMol trishydroxymethylaminomethane, pH 7.0.

[0021] Sodium 6-phosphogluconate reaction solution (R2 / 6PG)

[0022] Consists of 1 part of 400uMol sodium 6-phosphogluconate, 3 parts of 0.5mMol tris, pH 7.0.

[0023] R1 buffer

[0024] Consists of 1 part of 200uMol oxidized coenzyme II, 1 part of 117mMol magnesium chloride and 3 parts of 0.2mMol tris, pH 7.0.

Embodiment 2

[0025] The second of embodiment 2 kit composition

[0026] Component: R2 / G6P reaction solution

[0027] Consists of 1.5 parts of 300uMol glucose-6-phosphate sodium (G6P), 2 parts of 0.3mMol tris, pH 8.0.

[0028] R2 / 6PG reaction solution

[0029] Consists of 1.5 parts of 270uMol sodium 6-phosphogluconate, 2 parts of 0.3mMol tris, pH 8.0.

[0030] R1 buffer

[0031] Composed of 1.5 parts of 120uMol oxidized coenzyme II, 1 part of 117mMol magnesium chloride and 2 parts of 0.3mMol tris, pH 8.0.

Embodiment 3

[0032] Embodiment 3 The third of kit composition

[0033] Component: R2 / G6P reaction solution

[0034] Consists of 2 parts of 166uMol glucose-6-phosphate sodium (G6P), 1 part of 0.5mMol tris, pH 8.8.

[0035] R2 / 6PG reaction solution

[0036] Consists of 2 parts of 140uMol sodium 6-phosphogluconate, 1 part of 0.2mMol tris, pH 8.8.

[0037] R1 buffer

[0038] It consists of 2 parts of 84uMol oxidized coenzyme II, 1.5 parts of 117mM magnesium chloride and 1 part of 0.5mM trishydroxymethylaminomethane, pH 8.8.

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PUM

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Abstract

The invention discloses a red cell G6PD lack disease dual-enzyme ratio check method, a relative agent box and a relative method, belonging to blood check technical field. The invention checks the ascending speeds A and B when G6PD catalyzes G6P and 6GPD catalyzes 6PG of one sample at 340nm absorbance, to directly process judgment based on the ratio between A and B. The agent box is composed of R2 / G6P, R2 / 6PG, and R1 buffer liquids. And the method comprises that first preparing hemolysis liquid of object sample, processing test directly on a dual-channel automatic biochemical machine which can directly calculate out the ratio between A and B. The invention has accurate check, simple and quick operation, low cost, and wide application for checking G6PD lack disease.

Description

technical field [0001] The invention relates to blood detection technology, in particular to a double-enzyme ratio detection method for red blood cell G6PD deficiency, and also relates to a test kit for red blood cell G6PD deficiency and a method for using the kit. Background technique [0002] Patients with erythrocyte glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common genetic disease in my country, and the incidence rate is very high in the provinces south of the Yangtze River (about 3-16%). There are about 100 million patients with G6PD deficiency in my country. The disease is usually asymptomatic, but the patient's red blood cells are easily damaged by certain substances and hemolysis occurs, leading to hemolytic anemia, drug hemolysis, favism, etc. The most serious is neonatal hemolysis, which leads to kernicterus and develops into children. Lifelong mental retardation or death. Because the G6PD gene is located on the X chromosome, males have only one X chro...

Claims

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Application Information

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IPC IPC(8): G01N21/17G01N33/50
Inventor 杜琴
Owner 杜传书
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