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A set of fluorescent quantitative PCR primer and probe for detecting human boca virus

A Boca virus, fluorescent quantitative technology, applied in the field of microbial detection, fluorescent quantitative PCR primers and probes, to achieve high sensitivity and specificity

Inactive Publication Date: 2008-01-02
杭州致远医学检验所有限公司 +1
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  • Abstract
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  • Application Information

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Problems solved by technology

Currently, only Xiaoming Ma et al. used PCR technology to detect 318 clinical samples, and the positive detection rate was 5.7%.

Method used

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  • A set of fluorescent quantitative PCR primer and probe for detecting human boca virus

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Effect test

Embodiment 1

[0017] Example 1. Fluorescent quantitative PCR detection of Boca virus in children with lower respiratory tract infection

[0018] 1 Specificity of primers and probes

[0019] The specificity of primers and probes is the most important factor in the detection method established in this experiment. According to the sequence of human bocavirus in GenBank, primers and probes were selected to design NS1-specific genes, and compared by BLAST, the results showed that both the designed primers and the sequences of the detection probes had high specificity, which fully reached the detection level of human bocavirus. Boca virus requirements.

[0020] 2 The result of establishing the standard curve

[0021] The recombinant human bocavirus recombinant plasmid was diluted 10 times to 5.0×10 1 ~5.0×10 7 After 7 concentrations of copies / mL, quantitative PCR amplification was performed. The detection sensitivity can reach 5.0×10 1 Copy / mL, calculate the standard curve of the template c...

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Abstract

The invention discloses a fluorescent quantitative PCR primer and probe to detect human Boca virus in the microbe detecting technical domain (biological engineering product detetion), which is characterized by the following: designing the primer and probe according to the NC 007455 on the GenBank; making the length of oligonucleotide sequence between 20 and 30; displaying higher sensitivity and specificity; fitting for multiple facets based on the primer and probe, such as clinical disease diagnosis, antiviral drug sieving, environment monitoring and estimation, hygienic monitoring and port hygienic quarantine, epidemiologic survey and so on.

Description

technical field [0001] The invention belongs to the detection technology of microbes, especially the nucleic acid detection technology of lower respiratory tract infection human boca virus. The invention relates to a set of fluorescent quantitative PCR primers and probes for detecting human bocavirus, the sequence and application of the set of probes. Background technique [0002] Bocavirus (Human Bocavirus) is a new human parvovirus (Parvovirus) isolated recently from the secretions of children with lower respiratory tract infection. Doctors at the Tobias Allander Children's Hospital at the Karolinska Institutet in Stockholm, Sweden, reported the discovery while treating 540 children with lower respiratory tract infections. Among the children, 17 had the new virus, which Swedish doctors named "Bocavirus". Lower respiratory tract infections in children can be caused by different viruses, including respiratory syncytial virus, influenza virus, adenovirus, and coronavirus. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 杨宁敏林峰
Owner 杭州致远医学检验所有限公司
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