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Primer, probe and method for detecting human urological genital tract causal agent

A technology for urogenital tract and pathogens, applied in the field of primers and probes for detecting human urogenital tract pathogens, can solve the problems of high cost, unfavorable mixed infection detection, and time-consuming operation process, so as to reduce operation time, cost and Effects of Patient Financial Burden

Inactive Publication Date: 2008-02-06
SHANGHAI SHENYOU JIANHAI BIOLOGICAL TECH LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, at present, almost all clinically used PCR products are single detection, which can only detect one pathogen at a time, which is not conducive to the detection of mixed infections, and the operation process is relatively time-consuming and costly.

Method used

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  • Primer, probe and method for detecting human urological genital tract causal agent
  • Primer, probe and method for detecting human urological genital tract causal agent
  • Primer, probe and method for detecting human urological genital tract causal agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 detects the primers, probe sequences and methods of Neisseria gonorrhoeae (NG), ureaplasma urealyticum (UU), Chlamydia trachomatis (CT)

[0045] 1. Primers and Probes for Neisseria gonorrhoeae

[0046] According to the target nucleic acid sequence of Neisseria gonorrhoeae (Genbank Accession: M10316), suitable regions were selected to design primers and probes. Two sets of primers and probes were designed. After experimental comparison, one set was selected. The sequence is as follows:

[0047] NG-F1: 5'-GCT ACG CAT ACC CGC GTT GC-3' (SEQ ID NO: 1, located at target sequence 3141-3160bp)

[0048] NG-R1: 5'-CGA AGA CCT TCG AGC AGA CA-3' (SEQ ID NO: 2, located at target sequence 3531-3512bp)

[0049] The amplified products of NG-F1 and NG-R1 are 391bp long (see Figure 1), corresponding to the cryptic plasmid region of the target sequence.

[0050] Hybridization probe:

[0051] NG-P1: FAM-5'-CTG TTT AAG TCG TCC AGC TCG TTC-3'-BHQ1 (SEQ ID NO: 7, located at ...

Embodiment 2

[0087] Embodiment 2 detects the preparation of the kit of Neisseria gonorrhoeae (NG), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT)

[0088] 1. The raw material that kit of the present invention adopts

[0089] (1) Primers and probes

[0090] The primers and probes of the present invention were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0091] (2) Other materials

[0092]The purified water complies with the requirements of the "Quality Control Standards for Main Raw and Auxiliary Materials of Biological Products in China". Supplied by Lomag Corporation. All chemical reagents are analytically pure or above, and all reagents have passed the inspection.

[0093] (3) Reference substance

[0094] 1) Negative control substance

[0095] purified water. The alternative test is qualified.

[0096] 2) Positive control substance

[0097] Known concentration of NG, UU, CT pathogenic microorganism bacterial liquid, known concentration of...

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Abstract

The present invention discloses a guiding object and a probe for testing the human urogenital tract pathogens, and a method for testing the human urogenital tract pathogens using the guiding object and the probe. The present invention can test three different pathogens including Neisseria gonorrhoeae, mycoplasma ureaplasma and chlamydia trachomatis in a same reaction tube simultaneously. The present invention not only can increase the early detection rate of the sexually transmitted diseases, but also can reduce the operating time of the medical personnel, lower the cost, and lighten the economic burden of the patient.

Description

technical field [0001] The invention relates to the detection of pathogens in human urogenital tract secretions, in particular to a primer, a probe and a method for detecting human urogenital tract pathogens. Background technique [0002] With the gradual deepening of my country's opening to the outside world, coupled with the influence of some people in society on the idea of ​​"sexual freedom" and ignorance of sexually transmitted diseases, new STD patients have emerged in some open cities and economically developed areas. The flow of people between various places is very frequent, and the occurrence and spread of sexually transmitted diseases are intensifying. For example, in 2004, a total of 809,550 cases of sexually transmitted diseases (excluding HIV / AIDS) were reported in 31 provinces (municipalities and autonomous regions) of the country, an increase of 10% compared with the previous year. [0003] According to the 2004 National STD Epidemic Analysis Report, Neisseri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 熊慧张爱民陈嘉铮谢立群
Owner SHANGHAI SHENYOU JIANHAI BIOLOGICAL TECH LIABILITY
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