Escherichia coli biochemistry identification method

A biochemical identification technology for Escherichia coli, which is applied in the field of microbiology, can solve problems such as complicated procedures, limited work for identification of Escherichia coli, and various projects

Inactive Publication Date: 2008-03-12
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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  • Abstract
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Problems solved by technology

[0004] The inventor believes that there are many items in the current identification method and the procedures are complicated, which limits the identification work of large quantities of Escherichia coli

Method used

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  • Escherichia coli biochemistry identification method

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Embodiment Construction

[0013] In the national 863 project undertaken by the applicant. 343 strains of fecal coliform were obtained by collecting human, pig, cow, and chicken feces, and 290 strains of Escherichia coli were obtained by using the improved identification method, which saved money and time.

[0014] Table 3. Identification results of Escherichia coli using the improved identification method

[0015] sample source

[0016] In order to verify the accuracy of the improved identification method, the inventors verified it with the most authoritative identification method at present—the 16SrDNA gene sequencing method. From the 290 strains of Escherichia coli that were identified, 8 strains of Escherichia coli that had been biochemically identified were randomly selected for 16SrDNA gene sequencing and identification. The similarity rate is more than 99%, and the phylogenetic tree is shown in the attached figure, which fully verifies the feasibility of the identification method.

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Abstract

A colon bacillus biochemical authentication method belongs to the technical field of microbiology omits urease, phenyl alanine dehydrogenase and xylose from prior colon bacillus authentication items on the premise of having no impact on authentication accuracy and simplifies the biochemical authentication method, thus reducing authentication procedures, saving cost and particularly adapting to authentication of large-scale colon bacillus.

Description

technical field [0001] The invention belongs to the technical field of microbiology. Background technique [0002] From the perspective of simplicity, economy, practicality and feasibility, traditional biochemical identification and detection methods are mostly used in the monitoring of Escherichia coli in my country at present. That is, on the basis of detecting coliform bacteria and fecal coliform bacteria, biochemical identification is carried out to identify whether it is Escherichia coli. Dong Xiuzhu, Cai Miaoying and others [0003] There are 13 biochemical identification items for Escherichia coli given in the "Common Bacteria System Identification Manual", as shown in Table 1. [0004] The inventor believes that there are many items and complicated procedures in the current identification method, which limits the identification work of large quantities of Escherichia coli. [0005] Table 1. Biochemical identification items of Escherichia coli before improvement ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50C12Q1/10
Inventor 王耀兵杨滴
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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