Probe array and process for producing the same
A probe array and array technology, applied in chemical instruments and methods, biochemical equipment and methods, laboratory containers, etc., can solve the problems of biological substances and surface treatment deterioration
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Embodiment 1
[0133] Examples are given below to describe the present invention in detail. In this example, the uniformity of the substrate surface treatment coating was compared when the surface was coated after the chemical operation (imparting water resistance by printing) and when the separator sheet was adhered after the surface coating. The comparison method is to calculate the coefficient of variation (CV: (standard deviation / average value) × 100 (%)) of the fluorescence intensity obtained by hybridization between the three substrates on each column along the partition wall, and determine the distance at which the CV is stable. The size of the effective area ratio of hybridization evaluates the coating uniformity. The hybridization effective area ratio was defined as (area of the array region capable of obtaining stable CV / design area of the array region)×100(%).
[0134] First, on a poly-L-lysine-coated glass substrate, as shown in FIG. 8 , 99 kinds of mouse-derived cDNAs were ...
Embodiment 2
[0144] This example is an example of confirming the effect of the depth of the array region on the fluorescence intensity of the hybridization result. For the separator sheet prepared in Example 1, adjust the type of surface waterproof treatment agent and the thickness of the double-sided adhesive layer and / or the thickness of the single-sided adhesive layer, with two kinds of water resistance (the contact angle of water is 70 ° and 110 ° ) to prepare separator sheets of various thicknesses. Except for using such a spacer sheet, the nucleic acid probe array of Example was prepared in the same manner as in Example 1, hybridization was performed, and fluorescence intensity was measured and digitized. The result is shown in Figure 12.
[0145] As can be seen from FIG. 12 , the depth of the array region (also the thickness of the spacer sheet) changes sharply in the fluorescence intensity around 250 μm. From this result, it can be seen that the depth of the array region is prefe...
Embodiment 3
[0147] This example is an example of confirming the effect of the water repellency of the hydrophobic region on the partition wall of the separator sheet on the fluorescence intensity of the hybridization result. For the separator sheets prepared in Examples, the type of surface treatment of the acrylic layer and the like were adjusted to prepare separator sheets having various water repellency (water contact angle). Except for using separator sheets with different water repellency, the same operations as in Example 1 were used to prepare the nucleic acid probe array of the example, perform hybridization, measure the fluorescence intensity, and digitize it. The results are shown in Figure 13.
[0148] As can be seen from FIG. 13 , the fluorescence intensity changes rapidly when the contact angle of water is about 60° to 70°. From these results, it can be seen that the water resistance of the separator sheet preferably has a water contact angle of 60° or more, more preferably ...
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