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Method for replacing liquid, method for extracting component by using it, composite container and automatic analyzer

A technology of automatic analysis device and composite container, which can be used in laboratory containers, specific-purpose bioreactors/fermenters, solid separation, etc., and can solve problems such as complex device structure.

Inactive Publication Date: 2008-04-30
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this method, it is necessary to arrange the magnetic body directly above the test tube. When this method is applied to an automatic analyzer, it is also necessary to arrange the absorption and discharge device and the stirring device directly above the test tube. The obstacle of the device, as a result, there is a problem that the device structure becomes complicated

Method used

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  • Method for replacing liquid, method for extracting component by using it, composite container and automatic analyzer
  • Method for replacing liquid, method for extracting component by using it, composite container and automatic analyzer
  • Method for replacing liquid, method for extracting component by using it, composite container and automatic analyzer

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Embodiment 1

[0070] The following reagent solutions a to e (manufactured by Toyobo Co., Ltd., trade name MagExtractor (registered trademark) reagent solution) were poured into the five liquid exchange containers (31, 32) of the cartridge shown in FIG. 3 as described above. , 33, 34, 35). Next, 100 µL of blood (whole blood) was added to the liquid exchange container 31 of the cartridge. Next, after sufficient mixing, as described below, the magnetic body is sequentially moved in the liquid exchange container 31 and the liquid exchange container 35 by the magnetic body to perform liquid exchange, and nucleic acid in the blood is extracted. Next, measure the absorbance at 260nm (absorption wavelength of nucleic acid) and 280nm (absorption wavelength of protein) of the above-mentioned reagent solution e with a spectrophotometer, and obtain the degree of nucleic acid purification (absorbance at 260nm / absorbance at 280nm) and nucleic acid concentration (recovery rate) . The results are shown i...

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Abstract

A method for replacing liquid existing around a magnetic body in which both transfer of remaining liquid to the next process and loss of the magnetic body can be prevented, and the structure can be prevented from being complicated when that method is applied to an automatic analyzer. A container (11) containing liquid (16) in which a magnetic body (15) is placed, a container (12) containing liquid (17), a member (13) intermediating the movement of the magnetic body, and a magnetic force body (18) are provided, wherein the magnetic force body (18) is arranged outside the container (11). The magnetic body (15) is moved on the inner wall face of the container (11) and led out from the liquid (16) in which a magnetic body is placed by moving the magnetic force body (18). Subsequently, the magnetic body (15) is moved from the inner wall face of the container (11) to the surface of the member (13) intermediating the movement of the magnetic body, thence moved on the surface of the member (13) intermediating the movement of the magnetic body to the inner wall face of other container (12) and the magnetic body (15) is introduced into the liquid (17) in the other container (12) thus replacing the liquid existing around the magnetic body (15).

Description

technical field [0001] The present invention relates to a liquid exchange method for exchanging liquid existing around a magnetic body, a component extraction method using the same, a composite container, and an automatic analyzer. Background technique [0002] Conventionally, in the fields of biology and chemistry, magnetic materials have been used as solid phases for binding specific substances, for example, to perform extraction or separation of the specific substances. Specific examples include extraction or isolation of cells, bacteria, germs, proteins, nucleic acids, and the like. [0003] If a magnetic body is used, steps such as centrifugation or filtration separation may not be necessary. For example, in the presence of a chaotropic agent, nucleic acid can be extracted from cells as follows by utilizing the property that nucleic acid adsorbs to silica and using a magnetic substance. First, prepare magnetic microparticles (magnetic beads) whose surface is covered w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/10B03C1/00G01N33/553C12M1/00
CPCG01N35/0098B03C1/284B03C2201/18B03C1/286G01N33/54333B01L3/502B01L2200/0647B01L2300/0803B01L2300/0809B01L2300/087B01L2400/043
Inventor 和泉泽裕司
Owner ARKRAY INC
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