Method for replacing liquid, method for extracting component by using it, composite container and automatic analyzer

Abstract

A method for replacing liquid existing around a magnetic body in which both transfer of remaining liquid to the next process and loss of the magnetic body can be prevented, and the structure can be prevented from being complicated when that method is applied to an automatic analyzer. A container (11) containing liquid (16) in which a magnetic body (15) is placed, a container (12) containing liquid (17), a member (13) intermediating the movement of the magnetic body, and a magnetic force body (18) are provided, wherein the magnetic force body (18) is arranged outside the container (11). The magnetic body (15) is moved on the inner wall face of the container (11) and led out from the liquid (16) in which a magnetic body is placed by moving the magnetic force body (18). Subsequently, the magnetic body (15) is moved from the inner wall face of the container (11) to the surface of the member (13) intermediating the movement of the magnetic body, thence moved on the surface of the member (13) intermediating the movement of the magnetic body to the inner wall face of other container (12) and the magnetic body (15) is introduced into the liquid (17) in the other container (12) thus replacing the liquid existing around the magnetic body (15).

Description

technical field [0001] The present invention relates to a liquid exchange method for exchanging liquid existing around a magnetic body, a component extraction method using the same, a composite container, and an automatic analyzer. Background technique [0002] Conventionally, in the fields of biology and chemistry, magnetic materials have been used as solid phases for binding specific substances, for example, to perform extraction or separation of the specific substances. Specific examples include extraction or isolation of cells, bacteria, germs, proteins, nucleic acids, and the like. [0003] If a magnetic body is used, steps such as centrifugation or filtration separation may not be necessary. For example, in the presence of a chaotropic agent, nucleic acid can be extracted from cells as follows by utilizing the property that nucleic acid adsorbs to silica and using a magnetic substance. First, prepare magnetic microparticles (magnetic beads) whose surface is covered w...

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Problems solved by technology

However, in this method, it is necessary to arrange the magnetic body directly above the test tube. When this method is applied to an automatic analyzer, it is also necessary to arrange the absorption and discharge device and the stirring device directly above the test tube. The obstacle of the device, as a result, there is a problem that the device structure becomes complicated

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