Southern magnolia total lactone extract and preparation method and application thereof

A technology of Magnolia grandiflorum and total lactones, which is applied in the application field of Magnolia magnolia total lactones in antitumor drugs, can solve the problems of staying, the actual production and application are still far away, and achieves the advantages of reduced loss, strong industrial applicability and simple process. Effect

Inactive Publication Date: 2008-06-04
BEIJING SUNHO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on Magnolia Magnolia still remains in the aspects of monomer component separation, structural identification and cell biological activity measuremen

Method used

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  • Southern magnolia total lactone extract and preparation method and application thereof
  • Southern magnolia total lactone extract and preparation method and application thereof
  • Southern magnolia total lactone extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of total lactones of magnolia: take 10kg of magnolia leaves, grind into coarse powder, soak twice with deionized water, soak for 24h and 12h respectively, filter, discard the water extract, dry the filter residue, infiltrate with 95% ethanol Extract by rolling 3 times, the amount of solvent is 8 times of the amount of medicinal materials, combine the ethanol extracts, concentrate under reduced pressure at 60°C to an extract, add 30% ethanol 3L to reflux, dissolve the extract into a suspension, and dissolve it with petroleum ether Extract three times, the amount of petroleum ether is 3000mL each time, combine the petroleum ether extract, recover the petroleum ether solvent under reduced pressure at 40°C to about 2000mL, back-extract the petroleum ether with 80% ethanol twice, each time 1000mL, combine the ethanol extract , merged into the alcohol-water mixed medicinal solution, concentrated under reduced pressure at 60°C, added 1L of 8% ethanol to make the...

Embodiment 2～5

[0025] Implementation 2 to 5 is the preparation of total sesquiterpene lactones, 2 and 3 are respectively the examples extracted with methanol and acetone, and the rest are the same as in Example 1; 4 and 5 are respectively the implementation of turning chloroform into ethyl acetate and diethyl ether Example, all the other are identical with embodiment 1.

Embodiment 6

[0027] Take 50g from the 210.35g of total sesquiterpene lactones prepared in the above example 1, dissolve in ethanol, mix the sample with 30-60 mesh silica gel, and weigh 1.5kg of silica gel (100-200 mesh) and put it into a glass column by dry method In the column, the inner diameter × length of the column is 80 × 100cm. The silica gel mixed sample is installed on the clean silica gel, and the sample is covered with a layer of clean silica gel and absorbent cotton. First, it is eluted with mobile phase petroleum ether-ethyl acetate 10:1 until The color of the eluent becomes light and ends when no desired components come down, and then eluted with petroleum ether-ethyl acetate 3:1, followed by UV, TLC, and HPLC detection, and collects parthenolide and coynelide. Fractions were combined, the solvent was recovered under reduced pressure at 50°C, and dried to obtain 21.9 g of refined total magnolia lactones. The content composition of parthenolide and coyneolide measured by HPLC ...

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Abstract

The invention relates to a yulan magnolia total lactone extract, the preparation method and application thereof. Yulan magnolia leaves and/or flower is extracted with ethonal after being soaked in water; crude lactone can be obtained by low polarity organic solvent extract; the crude lactone is treated with the silica-gel column chromatography to obtain the extract of the invention. The invention has simple and feasible technique, definite components of the extract, and is suitable for industrialized production. The yulan magnolia total lactone in the invention has anti-tumor function, and can be used for preparing drugs with anti-tumor effects.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of active ingredients of natural medicines, and in particular relates to a process for extracting magnolia total lactones from Magnolia glabrata, and also relates to the application of magnolia total lactones in antitumor drugs. Background technique [0002] Magnolia grandiflora (Magnolia grandiflora L.), also known as lotus Magnolia, is a plant of the genus Magnolia in the family Magnoliaceae, an evergreen tree. Magnolia magnolia originates in the southeastern part of North America and was introduced to my country in the late 19th and early 20th centuries. It is currently widely cultivated in the Yangtze River Basin and cities to the south. Magnolia magnolia is a positive tree species, light-loving, semi-shade-tolerant, and is an excellent greening ornamental tree species. It has strong resistance to SO2, chlorine, etc., and its leaves are less used as medicine. It is mainly used for hyp...

Claims

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Application Information

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IPC IPC(8): A61K36/575A61P35/00A61K127/00A61K133/00
Inventor 张树祥熊国裕
Owner BEIJING SUNHO PHARMA
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