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Method for preparing laxogenin by smilax scobinicaulis plants

A technology of laxogenin and smilax nigranin, which is applied in the field of natural products, can solve the problems of low yield, high growth cost, complex technology of chemically synthesizing laxogenin, etc., and achieves low energy consumption, simple preparation process, Easy-to-master effect

Inactive Publication Date: 2008-06-25
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis of laxogenin is co

Method used

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Examples

Experimental program
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Effect test

specific Embodiment 2

[0027] Take 1 kg of crushed and dried Smilax chinensis tuber root, add 5L of methanol solvent, extract 3 times under reflux in a water bath at 60°C, 3 hours each time, extract 3 times, combine the extracts, dissolve the extract with deionized water, and put it in DIAION HP-20 Carry out purification on the macroporous resin, load the sample according to the ratio of sample to packing 1:6, pack the column, elution with 70% methanol (V / V) solution to remove impurities, collect the 80% methanol eluate, and dry to obtain 2.8g total saponins . Dissolve the total saponins in 15 mL at a concentration of 1 mol / L H 2 SO 4 (MeOH:H 2 (O=1:1) solution, 80 ° C hot water bath reflux hydrolysis for 1 h, after the reaction solution is cooled, concentrate under reduced pressure to remove methanol, then extract with cyclohexane, recover the solvent, N 2 1.4 g of Laxogenin was obtained by drying, with a purity of 96.6%.

specific Embodiment 3

[0028]Take 1 kg of crushed and dried Smilax smilax stems, add 4L of acetone, heat and reflux in a water bath at 65°C to extract 3 times, each time for 2 hours, extract 3 times in total, combine the extracts, recover acetone under reduced pressure, concentrate into an extract, and extract Dissolve in pure water, extract with an equal volume of petroleum ether, chloroform, and n-butanol for four times in turn, and recover the solvent under reduced pressure to obtain the extract. The chloroform extract was purified by DIAION HP-20 macroporous resin. Firstly, the column was washed with water, and the 90% methanol solution eluate was collected. After the eluate was concentrated and dried, it was purified by a Sephadex LH-20 column of dextran gel. According to chloroform: The eluent was eluted with methanol=1:1, and the eluate was collected and dried to obtain 0.5 g of Laxogenin with a purity of 96%.

[0029] Lasiogenin can be identified by its physicochemical properties and mass or...

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Abstract

The invention discloses a method for preparing the laxogenin sapogenin from the smilax scobinicaulis plants. The smilax scobinicaulis is extracted by utilizing the solvents and the extracts are dissolved in the water; then the obtained solutions are subject to the extraction using the weak-polarity and moderate-polarity organic solvents and water-immiscible C<4-10> lower alcohol solvents in order so as to obtain the extracts. The pure sapogenin is obtained in two ways that: one is to take the weak-polarity or moderate-polarity solvent extracts into the separation by the non-polar or weak-polar macroporous resin and obtain the laxogenin sapogenin by taking the isolates into the Sephadex LH-20 purification and drying the obtained mixtures; the other is carried out in the way that the lower alcohol extrats are taken and the total saponins are prepared through the weak-polar or polar macroporous resin; the diluted acids are added in the total saponins so as to carry out the hydrolysis and then the obtained solution is extracted using the weak-polarity solvents and the laxogenin sapogenin is obtained by drying the extracts. The method for preparing the laxogenin sapogenin has a simple process, delivers an easy-to-master technology, has small energy consumptions, high extraction rate, excenllent purity equotient of laxogenin sapogenin and low production cost, and provides raw materials for developing the drugs for treating the cardiovascular diseases and antitumor drugs, plant hormone products and chemical synthesis.

Description

technical field [0001] The invention relates to a method for preparing laxogenin from smilax blackthorn plant, which belongs to the field of natural products. Background technique [0002] Black-thorned smilax (Smilax scobinicaulis C.H.Wright), also known as short-stalked smilax, is a plant of the genus Smilax in the family Smilax. It grows in low mountain shrubs or valley river banks. It is mainly produced in Shaanxi and Gansu provinces, and is widely distributed in northern regions. The resources are very rich. Its rhizome is used as medicine instead of Clematis in northern my country, and it is called "wire clematis", "King Kong thorn", "King Kong vine" and so on. It has the effects of removing rheumatism, promoting blood circulation and detoxification, suppressing convulsions and wind, and anti-cancer. It is clinically used to treat rheumatic lumbar and leg diseases, infantile convulsion enteritis, scrofula, boils, cancer and other diseases. In the systematic separatio...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K36/896
Inventor 张存莉朱玮金玉鑫管桦兰光苏宝锋王熠
Owner NORTHWEST A & F UNIV
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