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Nano gold catalysis resonance scattering spectrometry for measuring grape-sugar

A technique of resonance scattering and glucose, which is used in the measurement of scattering properties, fluorescence/phosphorescence, and analysis by chemically reacting materials to achieve the effects of good selectivity, convenient operation and low detection limit.

Inactive Publication Date: 2008-07-23
GUANGXI NORMAL UNIV
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Problems solved by technology

So far, there has been no report on the detection of glucose by nano-gold catalyzed resonance scattering spectroscopy

Method used

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  • Nano gold catalysis resonance scattering spectrometry for measuring grape-sugar

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Embodiment

[0028] detection 1 # Sample (Ziguang Guhan Group Hengyang Pharmaceutical Co., Ltd.);

[0029] 2 # Sample (Liuji Industrial and Mining Group Dahua Pharmaceutical Co., Ltd.);

[0030] 3 # Sample (Zhengzhou Lingrui Pharmaceutical Co., Ltd.);

[0031] The glucose concentration of 3 different glucose injection samples, the operation steps are as follows:

[0032] 1. Prepare a test system with known glucose concentration:

[0033] (1) In a 5mL stoppered colorimetric tube, pipette 30μL of 69mg.mL in sequence -1 Copper sulfate solution, 110μL 0.25g.mL -1 Fehling's reagent A (calculated as sodium hydroxide), 100μL 47.8μg.mL -1 Gold gel, 50μL~120μL of 1mg.mL -1 Glucose, 30μL 60mg.mL -1 Potassium bromide solution, dilute to 2.5mL, react in a 75°C water bath for 9min;

[0034] (2) After the reaction, put the test tube into cold water and cool to room temperature to terminate the reaction;

[0035] (3) with the Cary Eclipse fluorescence spectrophotometer (Varian company of the U...

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Abstract

The invention discloses a nanometer gold catalysis resonance scattering spectrum method for testing amylaceum, which is based on the catalysis of nanometer gold on the copper bromide particles generated by Cu(II)-amylaceum-KBr, and the linear relationship between amylaceum in some density and delta I610nm. The invention has the advantages that compared with prior art, the invention combines catalysis reaction with resonance scattering spectrum technique, with high analysis sensitivity, low check limit, ug / mL level, better selectivity, simple operation, simple device, easily accessible agents and low cost, which only needs fluorescence spectrometer.

Description

Technical field: [0001] The invention relates to a method for measuring glucose, in particular to a nano-gold catalyzed resonance scattering spectrometry for measuring glucose. Background technique: [0002] Glucose is an indispensable substance in life activities. It can directly participate in the metabolic process in the human body. In the digestive tract, glucose is easier to be absorbed than any other monosaccharide, and after being absorbed, it can be directly used by human tissues. It has the functions of replenishing body fluids, Supply energy, replenish blood sugar, strengthen the heart and diuresis, promote detoxification, and also have a certain therapeutic effect on cancer. In addition, glucose is also used in printing and dyeing leather, mirror industry and thermos tank silver plating process. At present, the detection methods of glucose mainly include high performance liquid chromatography, enzyme catalysis, Fehling method and so on. The first two methods or ...

Claims

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Application Information

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IPC IPC(8): G01N21/75G01N21/64G01N21/47
Inventor 廖献就温桂清蒋治良
Owner GUANGXI NORMAL UNIV
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