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Automatic sorting method and apparatus for flow type cell art

A flow cytometry and automatic classification technology, applied in the field of automatic classification and statistical methods and devices of flow cytometry, can solve the problems of low efficiency, easy deviation of particle classification, etc. The effect of reducing the amount of calculation

Active Publication Date: 2008-07-23
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Abstract
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Problems solved by technology

[0006] The main purpose of the present invention is to provide an automatic classification method and device for flow cytometry, which can automatically classify particles accurately and efficiently

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  • Automatic sorting method and apparatus for flow type cell art
  • Automatic sorting method and apparatus for flow type cell art
  • Automatic sorting method and apparatus for flow type cell art

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Embodiment Construction

[0041] The present invention is suitable for flow cytometers, blood analyzers, urine analyzers and other particle analyzers based on flow cytometry. By analyzing and processing the collection of two-dimensional or multi-dimensional data of all particles flowing through, a certain The particles are classified into a certain class, and finally the particles are classified into the class that the sample should have.

[0042] Flow cytometry classification statistics system is generally as follows Figure 4 As shown, it includes: a sample generation device 2 , a light irradiation device 1 , a detector 3 and a classification statistics processor 4 . The sample generating device 2 further includes an interconnected gas-liquid transmission control module 22 and a flow chamber 21, the gas-liquid transmission control module 22 is used to make the sample fluid containing the cells or particles to be tested wrapped by the sheath fluid and pass through the flow chamber 21. The flow chambe...

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Abstract

The invention discloses an automatic classifying method of flow cytometry and a relative device, which comprises A1, according to at least two paths of light signal generated by one-by-one passing collected each cell or particle through illuminated region, representing each cell or particle into one vector with at least two dimensions and relation with the light signal strength of each path, B1, calculating two-two distances of all effective cells or particles, while the similarity of two cells or particles is higher along with the distance reduction, C1, aggregating the cells or particles with high similarity into one class, D1, repeating the step C1, at least aggregating all effective cells or particles into classes not lower than L according to the measurement theory. The invention canaccurately, effectively and automatically classify particles.

Description

【Technical field】 [0001] The invention relates to a method and a device for classifying and counting particles, and is especially suitable for an automatic classifying and counting method and device for flow cytometry. 【Background technique】 [0002] Flow cytometry and flow cytometry-based blood analyzers, urine analyzers, and particle analyzers all identify different particles in a liquid by collecting and analyzing two-dimensional or multi-dimensional data of particles to divide them into different categories. Such as figure 1 As shown, in the flow cytometer, the suspension of cells or particles 102 passes through an illumination area one by one under the sheath fluid wrapping, and in the illumination area, the particles 102 are irradiated by the laser light 101 to generate different optical signals, as described above The forward scattering signal FSC 104, the side scattering signal SSC 106, and the multi-channel fluorescent signal FL, etc., for example, 107 in the figu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49G01N15/10G06F19/00
CPCG01N33/5005
Inventor 易晗平楚建军顾问
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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