Cultivation method for wheat hybridization with nuclear male sterility

Abstract

The invention relates to a cultivating method of nuclear male sterile hybrid wheat, belonging to the field of crop cultivation. Prior method adds the 4E chromosome of Agropyron elongatum to a recessive genic male sterile wheat; the seed set of the acquired blue marked wheat genic male sterile maintainer line ranges from 40% to 50%; the white male sterile seed separated from self-crossed progeny is only around 64%. The invention is characterized in that, hybridization is carried out by taking lanzhou genic male sterile wheat as female parent and Agropyron elongatum as male parent; through light blue line self bred or white, light blue and dark blue three-color mixed line plant mixing (inbred + outcross), white sterile line and light blue fertile line seeds are bred; a photoelectric color sorter is used to sort the bred and produced white seeds and blue seeds, and in the blue seed group, the dark blue seeds are sorted and eliminated as much as possible; white sterile line and male parent line are hybrid to produce commercial hybrid F1 seeds. The cultivating method has the advantages that, the acquired light blue line has a high self seed set above 85 %, and the self seed set of white sterile line seeds can achieve around 80%.

Description

technical field [0001] The invention relates to the field of crop cultivation, in particular to a method for cultivating hybrid wheat. Background technique [0002] Compared with the "three-line" hybrid vigor utilization system of crop cytoplasmic male sterility, there is no special recovery and maintenance relationship for crop nuclear male sterility. The parental materials of excellent sterile lines, maintainer lines and male lines are extremely abundant, which is conducive to the breeding of strong dominant hybrid combinations. However, it is difficult to achieve effective maintenance of nuclear sterility by conventional methods, that is, it is difficult to produce a large number of homozygous sterile line seeds. Using the theory of wheat cytogenetics and wheat chromosome engineering technology to design a special cytogenetic mechanism, the effective maintenance of the GMS line can be realized. The principle of heredity and breeding is: in the homozygous male sterile ge...

AI Technical Summary

Problems solved by technology

However, since the 5R chromosome can be transmitted through pollen at a certain transmission rate in the Y line, at the same time, the mismatch between the two 5R chromosomes in the X line often occurs

For this reason, Driscoll (1985) later proposed an improved XYZ system, which replaced the 5R chromosomes in the original system with 5R isochromosomal chromosomes, improved the outer edge chromosomes in the system, and reduced the transmission rate through the male gamete pathway, but still can transmission, which means that the system is still unable to produce high homozygous GMS seeds, thus limiting its production application

In addition, the distinction between fertile and sterile is realized based on the fluff on the ear stem of the plant (fertile line Y) and no fluff (sterile Z line). This needs to be done after heading, thus increasing the number of sterile lines. reproductive cost

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