Tunny head immunological activity substance and method of preparing the same

Abstract

An immunoactive substance extracted from tuna fish head and a preparation method thereof are provided. The method comprises the following steps: pretreatment, washing, crashing, protease digestion, hydrolysis with neutral protease and papain, oil-water separation, hydrolysate centrifugation, oil-water separation of filtrate, separation of protein hydrolysate and fish oil, fractional separation, and separation of protein hydrolysate by using ultrafiltration membrane, to obtain immunoactive substance of tuna fish head. The invention utilizes two proteases to hydrolyze proteins of tuna fish head and centrifugally separates peptide-rich protein hydrolysate with immunological activity. The protein hydrolysate can be further processed to oral liquid, capsules and tablets. Furthermore, the invention produces fish oil rich in polyunsaturated fatty acids, thereby realizing the comprehensive and high-efficiency utilization of tuna fish head. The immunoactive substance of tuna fish head can significantly improve humoral immunity and nonspecific immunity in mice and enhance the cellular immune function of mice.

Description

technical field [0001] The invention relates to the fields of biotechnology application and comprehensive utilization of fish resources, in particular to a tuna head immunoactive substance and a preparation method thereof. Background technique [0002] Tuna (Tunas), also known as tuna and tuna, is distributed in the vast waters of the Pacific Ocean, the Atlantic Ocean and the Indian Ocean. It is an oceanic highly migratory fish. The fish is large and heavy. Most tuna live in sea areas with a depth of 100-400 meters, and their migratory range is limited to the high-salinity ocean, and they rarely come into contact with seriously polluted offshore and coastal waters. Tuna is a kind of deep-sea fish, which has always been famous in the international market for its high nutritional value, pure natural, and pollution-free, and is known as "ocean gold". Tuna is an important resource of pelagic fisheries in the world. The annual output of tuna in the world has remained at 3.5-4 mi...

AI Technical Summary

Problems solved by technology

At present, there is still a lack of effective processing and utilization methods. Many companies at home and abroad process tuna scraps into feed fish meal, and many companies directly treat them as waste, which wastes resources and causes environmental pollution.