Preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and preparation method of corresponding tablet thereof

Abstract

A preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and corresponding tablets comprises the following steps: mixing a raw material of mussel dry powder with a buffer according to a weight volume ratio of 1:20-1:25, homogenizing the mixture, adjusting the pH value to 9.5-10 to obtain a mixed solution; heating the mixed solution to 50-55 DEG C, preheating for 10-15 min, respectively adding an alkaline protease and a neutral protease with a weight being 1.0%-1.5% of the raw material, performing enzymatic hydrolysis at 50-55 DEG C for 2-4 hours; performing enzyme deactivation of the enzymatic hydrolytic product to obtain an enzymatic hydrolytic solution, then orderly performing desalination, concentration, and spray drying of the enzymatic hydrolytic solution to obtain the immune-enhancing enzymatic hydrolytic polypeptide. The invention also discloses a related immune-enhancing mussel polypeptide tablet. The preparation method of the invention is simple; the obtained immune-enhancing polypeptide and its tablet have high activity; the raw material resource is abundant; and the preparation method has strong industrial implementation potential.

Description

technical field [0001] The invention relates to a method for preparing an immune-enhancing mussel enzymolysis polypeptide and a corresponding tablet by enzymatically hydrolyzing molluscs such as mussels. Background technique [0002] Mussels belong to the phylum Molluscs, Phallobranches, Mytilidae, Mytilaceae, and the genus Mussels. They are marine bivalve molluscs, commonly known as mussels and sea reds. They are known as "eggs in the sea". It needs 8 kinds of essential amino acids such as valine and leucine, and the content is much higher than that of eggs, chicken, duck, fish, shrimp and meat. [0003] The excellent protein composition of mussels provides a guarantee for the in-depth development of mussels. For example, New Zealand extracts medicines for treating rheumatoid arthritis and osteoarthritis from mussels, and Russia uses mussels to produce an anti-atherosclerosis food. However, mussels in our country basically stay in fresh sales without shells, and are proces...

AI Technical Summary

Problems solved by technology

The above methods use the enzymatic hydrolysis process to prepare polypeptides with specific efficacy, but due to the relatively low efficiency of single enzyme enzymatic hydrolysis, the yield of polypeptides with specific efficacy is extremely low, and it is difficult to meet the needs of industrialization

In addition, the Chinese patent CN 101991840A "Preparation method of mussel active polypeptide" studies a method of preparing mussel active polypeptide by using a vacuum ultrasonic reactor under acidic conditions. Although this method has the advantages of simple process and suitable for industrial

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