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Preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and preparation method of corresponding tablet thereof

An immune-enhancing and mussel enzyme technology, applied in food preparation, application, pill delivery, etc., can solve the problem that the preparation process and formula of immune-enhancing polypeptide tablets have not been reported, the preparation cannot be directly used with high purity requirements, and a single enzyme enzyme is required. Solve problems such as low relative efficiency, and achieve the effect of significant immune enhancement, good health care, and no toxic side effects

Active Publication Date: 2013-03-06
ZHEJIANG OCEAN UNIV
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Problems solved by technology

The above methods use the enzymatic hydrolysis process to prepare polypeptides with specific efficacy, but due to the relatively low efficiency of single enzyme enzymatic hydrolysis, the yield of polypeptides with specific efficacy is extremely low, and it is difficult to meet the needs of industrialization
In addition, the Chinese patent CN 101991840A "Preparation method of mussel active polypeptide" studies a method of preparing mussel active polypeptide by using a vacuum ultrasonic reactor under acidic conditions. Although this method has the advantages of simple process and suitable for industrial production, However, the obtained enzymatic hydrolysis products contain not only polypeptides, but also polysaccharides and other substances, which are mixtures of various components, and the obtained preparations cannot be directly used in applications with high purity requirements.
[0005] During the search process, the applicant found that mussels were used as raw materials in my country, and the research on the preparation of immune-enhancing polypeptides by using enzymatic hydrolysis technology was still in a blank stage. The preparation technology and formula of tablet have not been reported yet

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  • Preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and preparation method of corresponding tablet thereof
  • Preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and preparation method of corresponding tablet thereof
  • Preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and preparation method of corresponding tablet thereof

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Embodiment Construction

[0028] Below in conjunction with embodiment the present invention is described in further detail.

[0029] A preparation process of mussel enzyme-hydrolyzed polypeptide for immune enhancement and tablet preparation method thereof, the preparation process is as follows: dry mussel powder → enzymatic hydrolysis → macroporous resin desalination → spray drying → immune enhancement peptide → tablet.

[0030] The specific steps are:

[0031] 1. Weigh 5.0g of mussel defatted dry powder, add 125mL of glycine-NaOH buffer solution with a pH of 10, homogenize in a tissue masher for 5min, adjust the pH of the mixture to 9.5 with 1mol / L HCl, and mix liquid.

[0032] 2. Raise the temperature of the mixture to 55°C, stir and preheat, and keep it warm for 20 minutes under stirring; then add 0.075g of alkaline protease (enzyme activity ≥ 1.9×10 4 U / g) and 0.050g neutral protease (enzyme activity ≥4.9×10 4 U / g) to start the enzymolysis reaction, the enzymolysis temperature is 53-55°C, stirri...

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Abstract

A preparation method of immune-enhancing mussel enzymatic hydrolytic polypeptide and corresponding tablets comprises the following steps: mixing a raw material of mussel dry powder with a buffer according to a weight volume ratio of 1:20-1:25, homogenizing the mixture, adjusting the pH value to 9.5-10 to obtain a mixed solution; heating the mixed solution to 50-55 DEG C, preheating for 10-15 min, respectively adding an alkaline protease and a neutral protease with a weight being 1.0%-1.5% of the raw material, performing enzymatic hydrolysis at 50-55 DEG C for 2-4 hours; performing enzyme deactivation of the enzymatic hydrolytic product to obtain an enzymatic hydrolytic solution, then orderly performing desalination, concentration, and spray drying of the enzymatic hydrolytic solution to obtain the immune-enhancing enzymatic hydrolytic polypeptide. The invention also discloses a related immune-enhancing mussel polypeptide tablet. The preparation method of the invention is simple; the obtained immune-enhancing polypeptide and its tablet have high activity; the raw material resource is abundant; and the preparation method has strong industrial implementation potential.

Description

technical field [0001] The invention relates to a method for preparing an immune-enhancing mussel enzymolysis polypeptide and a corresponding tablet by enzymatically hydrolyzing molluscs such as mussels. Background technique [0002] Mussels belong to the phylum Molluscs, Phallobranches, Mytilidae, Mytilaceae, and the genus Mussels. They are marine bivalve molluscs, commonly known as mussels and sea reds. They are known as "eggs in the sea". It needs 8 kinds of essential amino acids such as valine and leucine, and the content is much higher than that of eggs, chicken, duck, fish, shrimp and meat. [0003] The excellent protein composition of mussels provides a guarantee for the in-depth development of mussels. For example, New Zealand extracts medicines for treating rheumatoid arthritis and osteoarthritis from mussels, and Russia uses mussels to produce an anti-atherosclerosis food. However, mussels in our country basically stay in fresh sales without shells, and are proces...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L1/305A61K38/01A61K9/20A61P37/04
Inventor 王斌罗红宇马佳卉栗丽
Owner ZHEJIANG OCEAN UNIV
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