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Cynoglossus semilaevis special numerator mark and uses thereof

A semi-smooth tongue sole and molecular marker technology, applied in the molecular biology of fish genetic sex identification, fish molecular markers and genetic sex identification, can solve the problems of no fish female sex-specific molecular markers found, no reports, etc.

Inactive Publication Date: 2008-09-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current research has only found a few male sex-related molecular markers in some fish, but has not found fish female sex-specific molecular markers
However, there are no reports about sex-specific molecular markers and genetic sex identification of half-smooth tongue sole at home and abroad.

Method used

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  • Cynoglossus semilaevis special numerator mark and uses thereof
  • Cynoglossus semilaevis special numerator mark and uses thereof
  • Cynoglossus semilaevis special numerator mark and uses thereof

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Experimental program
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Embodiment Construction

[0092] figure 1 Among them, the underline "=" indicates the sequence of AFLP primer; the underline "—" indicates the sequence of the female-specific primer used in genetic sex identification PCR.

[0093] figure 2 middle,

[0094] A-1 and A-2 are the results of amplification using CseF783N1 and CseF783C1

[0095] B-1 and B-2 are the results of amplification using CseF464N1 and CseF464C1;

[0096] C-1 and C-2 are the results of amplification using CseF136N1 and CseF136C1.

[0097] ♀ represents a female individual; Indicates a male individual.

[0098] The present invention adopts modern molecular biology technology to screen out sex-specific molecular markers and establish molecular technology for genetic sex identification of tongue sole, which is useful for cultivating all-female seedlings of tongue-sole half-smooth sole, raising all-female seedlings of tongue-sole half-smoothness sole, increasing the breeding output of tongue-sole half-smooth sole, and improving bree...

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Abstract

A female specific molecular marker of tongue sole and application thereof comprises a screening method, DNA extraction of the tongue sole genomic, AFLP analysis of genomic DNA and screening of female specific molecular marker; also comprises clone and sequence of the female specific molecular marker of tongue sole, recycle of the female specific molecular marker of tongue sole, clone of female specific molecular marker and sequence of female specific molecular marker; additionally comprises application of one kind of female specific molecular marker for applying on the uninjurous identification of genetic sex of tongue sole, i.e. the genomic DNA of the tongue sole to be determined is collected, three pairs of specific primer are respectively designed according to the sequence of the female specific molecular marker of three tongue sole obtained, any pair of these three pairs of female specific primer is adopted to do PCR amplification, therefore the identification of genetic sex of tongue sole can be carried out. The female specific molecular marker of tongue sole and application thereof has the advantages of the advancement, the high efficiency, the accuracy and the reliability, having the important application value on the unisexual fingerlings production of the tongue sole and the wide application prospect on the genetic sex identification and the sex control research of other fishes.

Description

technical field [0001] The invention belongs to fish molecular markers and genetic sex identification technology in the technical field of aquatic organisms, and is a molecular biology method for fish genetic sex identification that can be applied in fish parthenogenesis. Background technique [0002] my country is rich in fish resources, many of which have gender differences in growth rate. For example, male tilapias grow about 40% faster than females, while seawater fish such as flounder and half-smooth tongue sole, females grow faster than males. Individual growth is 40%-100% faster. Therefore, research on genetic sex detection and sex control of these fishes has important scientific significance, and has great application potential and promotion prospects. [0003] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal areas of my c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/10C12N15/12
Inventor 陈松林马洪雨李静邓思平田永胜沙珍霞
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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