ATP motor rotary type biosensor single molecule analysis and separation technology and method

Abstract

With the aid of basic researches on ATP molecular motor structures and functions, the invention further designs a full-circle biological sensor which is used for analyzing unimolecules that are taken as carrier connecting locus of Epsilon subunits, a preparation method of a plurality of CdTe semiconductor nano crystals that have pH sensitivity, water solubility and high photoluminescence efficiency, and a full-circle ATP motor sensor which is used for marking charge free bodies of ATP motor compounds in a single direction. By adopting the compound that is obtained by combining the full-circle biological sensor with the molecular motor principle and the quantum dot technique, the invention generates a unimolecule device that has novel functions, and combines biological molecule analyzing and separating functions with high resolution capability into a whole; the invention can be developed into a novel analyzing and separating technique with ultra-high resolution in physics, can be widely used in multiple fields of environment, national defense, energy and information, etc., thus having wide application value and enormous economic and social benefits.

Description

technical field [0001] The invention belongs to the field of ultra-high sensitivity single-molecule optical coding analysis and single-molecule separation technology of nanobiology, and relates to the design and assembly of single-molecule single-molecule of biosensors with full-rotation ATP motors, as well as the importance of new methods for the synthesis of pH-sensitive CdTe semiconductor quantum dot labels. Improved, novel applications of quantum dot-labeled surface charge isolates of the ATP-motor complex in optically encoded assays and their separation. Background technique [0002] Last year, American scientists such as Roukes developed a research result of 7,000 Dalton resolution by using the principle of very high-frequency physical vibration; for this reason, a scientific magazine commented that their results "pull down the impossible obstacle to overcome". Since then, scientists have started A new round of scientific competition in the direction of the development...

AI Technical Summary

Problems solved by technology

However, there are obvious shortcomings in this technology, which requires a distance from the application under extremely low temperature conditions.

[0003] At present, the analysis and separation technology of biological samples mainly uses the expensive fluorescence device of flow cell separation technology, and the resolution is mainly based on cells (30uM); the analysis of single-molecule nanoparticles uses confocal combined with a special device for microfluidic channels. Device analysis of microRNAs has also been reported by Neely et al., Nat Methods 3(2006) 41-46.; but all the above-mentioned technologies are far from the ultra-high resolution requirements of current scientific research (reaching Dalton resolution)

Images