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Manufacturing process of a thin-layer electrode for pesticide residue biosensor

A technology for biosensors and pesticide residues, applied in the field of biosensors, can solve problems such as complex preparation processes, and achieve the effects of good reproducibility, low cost, and a wide range of applicable voltages

Inactive Publication Date: 2011-12-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Biosensors are an important part of electrochemical detection methods. Traditional biosensor electrodes use screen printing technology. The preparation of electrodes requires professional printing materials and equipment, and the preparation process is complicated.

Method used

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  • Manufacturing process of a thin-layer electrode for pesticide residue biosensor
  • Manufacturing process of a thin-layer electrode for pesticide residue biosensor
  • Manufacturing process of a thin-layer electrode for pesticide residue biosensor

Examples

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specific Embodiment

[0022] (1) The preparation of silver-plated thin-layer basic electrodes and leads is as follows: figure 1 As shown, a layer of PVC insulating tape [2] is pasted on the ordinary glass slide [1], and then a layer of mask with the shape of the reserved thin layer of the basic electrode and its lead wire and the lead wire of the working electrode is pasted on the insulating tape. Make a template, scrub the template with absolute ethanol, rinse it with distilled water and dry it, then soak it in 1mol / L sodium hydroxide solution for 10 minutes, then rinse it with distilled water, dry it for later use, silver nitrate solution, hydroxide The proportioning of sodium solution, ammonia water, glucose solution silver mirror reaction liquid is: 1.5ml 2% silver nitrate solution, 1 drop 5% sodium hydroxide solution, 13 drops 2% ammonia water, 9 drops 10% glucose solution, every template 0.35ml of reaction solution was added dropwise, and the silver mirror reaction was repeated 9 times on the...

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Abstract

The invention discloses a thin-layer electrode of a pesticide residue biosensor and its manufacturing process, including: preparation of a silver-plated thin-layer basic electrode and its lead wire, preparation of a silver / silver chloride reference electrode, preparation of a carbon paste working electrode, etc. The process is made. Compared with the prior art, the invention has the advantages of simpler manufacturing process, lower cost, good reproducibility, wide applicable voltage range and the like.

Description

technical field [0001] The invention relates to a biosensor electrode, in particular to a pesticide residue biosensor thin-layer electrode and a preparation process thereof, belonging to the technical field of biosensors. Background technique [0002] People pay more and more attention to food safety and agricultural environment problems caused by pesticide residues and their pollutants. Measures are being taken at home and abroad to speed up the research on pesticide residue detection technology. In recent years, the use of biosensor technology to detect pesticide residues has attracted attention. The principle is to indirectly detect the residues of these pesticides by detecting the relevant physical and chemical properties of the intermediates produced after the pesticides are degraded. Nitrobenzene compounds are commonly used intermediates for the synthesis of organophosphorus pesticides, and are widely present in petrochemical, dye, soap, pharmaceutical and other waste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 郭康权项方献
Owner NORTHWEST A & F UNIV