Supercharge Your Innovation With Domain-Expert AI Agents!

Method of deriving progenitor cell line

A progenitor cell line, cell line technology, applied in embryonic cells, animal cells, vertebrate cells, etc., can solve the problems of harmful effects and inefficiency of tissue repair and regeneration

Inactive Publication Date: 2009-01-07
AGENCY FOR SCI TECH & RES
View PDF28 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this special property of embryonic stem cells also poses unique challenges, that is, it is necessary to produce sufficient and homogeneous cell types for the treatment of specific diseases or tissue injuries to ensure therapeutic efficacy, and to inhibit the production of cells that are critical for tissue repair and tissue damage. Regenerate other cell types that have deleterious effects
Current methods that either enhance differentiation of embryonic stem cells to specific cell lines and / or enrich specific tissue cell types are too inefficient and generally produce heterogeneous cell populations that lead to tumorigenesis (Keller, Genes Dev. 2005;19:1129- 1155; Wobus and Boheler, Physiol Rev. 2005; 85:635-678)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of deriving progenitor cell line
  • Method of deriving progenitor cell line
  • Method of deriving progenitor cell line

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0115] It is believed that the methods described herein enable the preparation of progenitor cells, which are of course differentiated using methods known in the art. Thus, any use of differentiated cells will apply equally to those progenitor cells from which they were derived.

[0116] The progenitor cells and combinations prepared by the method described herein can be used for the treatment of diseases, or for preparing pharmaceutical compositions for treating diseases. The disease can include diseases treated by regenerative therapies, including heart failure, bone marrow disease, skin disease, burns, and degenerative diseases such as diabetes, Alzheimer's disease, Parkinson's disease, and cancer.

[0117] We thus disclose a method of disease treatment comprising (a) providing embryonic stem (ES); (b) constructing a progenitor cell line from the embryonic stem cell, wherein the progenitor cell line is selected based on its ability to self-renew; (c ) optionally obtaining ...

Embodiment

[0211] Example 1. Method: Obtaining E-RoSH cell lines

[0212] Embryonic stem cells (ESCs) were induced to differentiate into embryoid bodies (Ebs) using the methylcellulose-based method described by Lim et al., Blood. 1997; 90: 1291-1299.

[0213] Harvest the embryoid bodies of 3-6 days, separate them into a single cell suspension by collagenase digestion (see Robertson EJ, ed. Teratocarcinomas and embryonic stem cells: apractical approach. Oxford: IRL Press Limited; 1987: 71-112) and Take 1-5×10 5 Cells / 10cm feeder layer culture plate density for planting. After about 1 week, the expressing cells differentiated into a complex mixture of various cell types.

[0214] Rapidly dividing cell clones resembling embryonic-derived RoSH cells were picked and expanded sequentially in 48-well plates, 24-well plates, 6-well plates, and subsequently in 10 cm plates. The cultures of the individual clones were sequentially named E-RoSH1, 2, 3..., and each culture was constructed in this ...

Embodiment 4

[0225] Example 4. Methods: In Vitro Endothelial Differentiation

[0226] Endothelial differentiation of acetylated LDL extracted from differentiated E-RoSH cells and E-RoSH cells was performed as described above (Yin et al, Arterioscler Thromb Vasc Biol. 2004; 24:691-696).

[0227] E-RoSH vascular structures differentiated in vitro were fixed in formalin, embedded in paraffin, sectioned at 4 μm, and vWF was stained with rabbit polyclonal antibody and Envision+System-peroxidase (DakoCytomaton, Gostrup, Denmark). Sections were counterstained with Mayer's hematoxylin.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

We disclose a method comprising: (a) providing an embryonic stem (ES) cell; and (b) establishing a progenitor cell line from the embryonic stem cell; in which the progenitor cell line is selected based on its ability to self-renew. Preferably, the method selects against somatic cells based on their inability to self-renew. Preferably, the progenitor cell line is derived or established in the absence of co-culture, preferably in the absence of feeder cells, which preferably selects against embryonic stem cells. Optionally, the method comprises (d) deriving a differentiated cell from the progenitor cell line.

Description

[0001] US Provisional Application Serial No. US60 / 713992 filed September 2, 2005 is incorporated by reference. [0002] The foregoing application, and this application and each document cited or referenced in the foregoing application, including the pendency of the foregoing application, and any of the foregoing applications, documents, and any documents cited in this application and documents How-to guides or catalogs for products are incorporated herein by reference. Furthermore, any document cited herein, and all documents cited or referenced in a cited document herein, as well as all operating instructions or catalogs for any product herein or incorporated herein, are hereby incorporated by reference into In this article. Documents or any teachings incorporated herein by reference may be used in the practice of the invention herein. Documents incorporated herein by reference are not admissions of prior art. technical field [0003] The invention relates to the fields of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08G01N33/50C12N5/0775
CPCC12N2501/115C12N2506/02C12Q1/6809G01N33/5044C12N2501/135C12N5/0662A61P17/00A61P17/02A61P25/16A61P25/28A61P35/00A61P35/02A61P9/00A61P3/10C12N5/0606C12N5/00G01N33/50C12N5/0603C12N2501/734C12N2502/1352
Inventor 林赛江埃利亚斯·赖
Owner AGENCY FOR SCI TECH & RES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com