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Method preparing methilanin and prosome homoserine or succinyl homoserine thereof using sulphate permease expression-enhanced tiny organism

A methionine, microorganism technology, applied in fermentation, bacteria and other directions, can solve problems such as toxicity

Active Publication Date: 2009-01-21
EVONIK OPERATIONS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Accumulation of homocysteine ​​is toxic to E. coli (Tuite et al., 2005 J. Bacterid, 187, 13, 4362-4371.), while having a negative regulatory effect on metA expression via MetR

Method used

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  • Method preparing methilanin and prosome homoserine or succinyl homoserine thereof using sulphate permease expression-enhanced tiny organism
  • Method preparing methilanin and prosome homoserine or succinyl homoserine thereof using sulphate permease expression-enhanced tiny organism
  • Method preparing methilanin and prosome homoserine or succinyl homoserine thereof using sulphate permease expression-enhanced tiny organism

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Embodiment Construction

[0133]PCT N° PCT / IB04 / 001901 filed 12 May 2004 describes an E. coli strain in which the methionine repressor encoded by the metJ gene is replaced by a chloramphenicol cassette (ΔmetJ::Cm) , and has the metA allele that responds to methionine and SAM (metA ＊ 11) Has reduced feedback sensitivity. The following genetic modifications were introduced into this strain.

[0134] MG1655metA ＊ 11 Construction of ΔmetJ::Cm Ptrc-metF::Km

[0135] To clone the metF gene under the control of a heterologous Ptrc promoter, the homologous recombination strategy described by Datsenko & Wanner (2000) was used. This strategy allows insertion of chloramphenicol or kanamycin resistance cassettes near the gene of interest. For this, the following oligonucleotides were used:

[0136] PtrcmetF R (SEQ ID NO 1)

[0137]

[0138] which has:

[0139] - Regions (uppercase) homologous to the sequence (4130259-4160195) of the gene metF (reference sequence on the website http: / / genolist.pasteur.fr / ...

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Abstract

The present invention relates to a process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism claimed is modified in a way that the production of cysteine and / or C1 units is enhanced and / or the transfer potential of the C1 units on 10 homocysteine is increased or optimized. The isolation of methionine or its derivates from the fermentation medium is also claimed.

Description

technical field [0001] The present invention relates to a method for the production of methionine or its derivatives by culturing microorganisms in a suitable medium comprising a carbon source and a sulfur source. The claimed microorganisms are modified in such a way that the production of cysteine ​​and / or C1 units is enhanced and / or the transfer potential of C1 units onto homocysteine ​​is increased or optimized. Also claimed is the isolation of methionine or its derivatives from the fermentation medium. Background technique [0002] Sulfur-containing compounds such as cysteine, homocysteine, methionine or S-adenosylmethionine are critical for cell metabolism and are produced industrially for use as food or feed additives and pharmaceuticals . In particular methionine, an essential amino acid that cannot be synthesized by animals, plays an important role in many bodily functions. In addition to its role in protein biosynthesis, methionine is also involved in transmethyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/12C12P13/06C12N1/21
CPCC12P13/12C12P13/06
Inventor 雷纳·菲格法比安·卢克斯塞利娜·雷诺米歇尔·沙托菲利普·苏卡勒
Owner EVONIK OPERATIONS GMBH
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