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Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass

A nucleotide sequence, biomass technology, applied in the field of transgenic plants, can solve the problem of time-consuming and laborious breeding process

Active Publication Date: 2009-02-18
CERES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the breeding process is time-consuming and labor-intensive

Method used

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  • Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass
  • Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass
  • Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0119] Preparation of soil mixture: 24L SunshineMix #5 soil (Sun Gro Horticulture, Ltd., Bellevue, WA) was mixed with 16L Therm-O-Rock vermiculite (Therm-O-Rock West, Inc., Chandler, AZ) in cement Mix in a cement mixer to prepare a 60:40 soil mixture. To this soil mixture was added 2 Tbsp Marathon 1% pellets (Hummert, EarthCity, MO), 3 Tbsp 14-14-14 (Hummert, Earth City, MO) and 1 Tbsp Peters fertilizer 20-20-20 (J.R. into the soil and mix thoroughly. Generally fill 4-inch diameter pots with the soil mix. The pots were then covered with 8-inch nylon membrane squares.

[0120] Planting: Aspirate 35 mL of seed mixture using a 60 mL syringe. Add 25 drops to each pot. A clear propagation dome was placed on top of the pot, and the pot was then placed under a 55% shaded weave and subirrigated by adding 1 inch of water.

[0121] Plant Culture: 3 to 4 days after planting, remove covers and shade braids. Water the plants as needed. After 7-10 days, use tweezers to thin the po...

Embodiment 1

[0204] Example 1: Lead28-ME04701-clone 1952-cDNA 13499809 (SEQ ID NO:90)

[0205] T 1 Qualitative analysis of plants:

[0206] All 10 events produced rosettes with more leaves and more inflorescences than the control. The plants were also slightly smaller than the controls (Table 1-1). A transgenic "control" is a group of plants expressing a different 35S::cDNA but indistinguishable from untransformed WS wild type. This method of scoring phenotypes is typical for our large-scale morphological phenotyping project.

[0207] Table 1-1. In 35S::cDNA 13499809 T 1 Qualitative phenotypes observed in the event

[0208] event The number of raised rosette leaves, the number of raised inflorescences & slightly smaller ME04701-01 x ME04701-02 x ME04701-03 x

[0209] ME04701-04 x ME04701-05 x ME04701-06 x ME04701-07 x ME04701-08 x ME04701-09 x ME04701-10 x

[0210] T 2 Quantitative analysis of plants...

Embodiment 2

[0226] Example 2: Lead 29-ME04717-clone 123905-cDNA 12562634 (SEQ ID NO:92)

[0227] Ectopic expression of Ceres cDNA 12562634 under the control of the 326D promoter induced a number of phenotypes, including:

[0228] · Increased number of inflorescences

[0229] • Continued emergence of rosette leaves after flowering, resulting in an overall increased leaf count.

[0230] • Abnormal expression of Ceres cDNA 12562634 can be applied to increase the number of branches and inflorescences. This can have a significant impact on the seed number.

[0231] T 1 Qualitative analysis of plants:

[0232] Using the 326D promoter, 9 out of 10 events produced rosettes with more leaves and more inflorescences than controls (Table 1). One of the nine events also had a fertility defect, very similar to that observed with 35S::cDNA12562634. A transgenic "control" is a group of plants expressing a different 35S::cDNA construct and indistinguishable from untransformed WS wild type. This met...

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Abstract

The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of modulated plant size, vegetative growth, organ number, plant architecture, growth rate, seedling vigor, growth rate, fruit and seed yield, tillering and / or biomass in plants. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, organ number, plant architecture, growth rate, seedling vigor and / or biomass that are altered with respect to wild type plants grown under similar conditions.

Description

technical field [0001] The present invention relates to isolated nucleic acid molecules and corresponding polypeptides encoded by them, which are capable of modulating plant growth rate, vegetative growth, organ size, architecture seedling vigor and / or biomass in plants. The present invention also relates to the use of nucleic acid molecules and polypeptides to produce transgenic plants having modulated growth rate, vegetative growth, organ number, architecture, seedling vigor and / or biomass compared to wild-type plants grown under similar conditions, Plant cells, plant material or plant seeds. Background of the invention [0002] Molecular techniques can be used to obtain plants specifically improved for use in agriculture, horticulture, biomass conversion and other industries (eg paper industry, plants as production plants for proteins or other compounds). For example, modulating the overall size of a plant, or the size of any of its organs, or the number of any of its or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00C12N15/79C12N5/14
CPCC07K14/415C12N15/8261Y02A40/146
Inventor N·亚历山德洛夫V·布罗沃P·麦斯西亚K·A·弗莱德曼C·克里斯坦森G·纳扎恩
Owner CERES INC
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