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Bacillus tubercle gene vaccine for treating medicine-tolerant pulmonary tuberculosis

A technology of Mycobacterium tuberculosis and genetic vaccines, applied in the direction of antibacterial drugs, bacterial antigen components, etc.

Inactive Publication Date: 2009-03-04
中国人民解放军总医院第二附属医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These three vaccines are dead vaccines, which can only induce humoral immunity and Th1-type cellular immune responses, but cannot induce specific cytotoxic T lymphocyte (CTL) responses
[0006] At present, there are no research reports on the application of nucleic acid vaccines in the treatment of drug-resistant tuberculosis at home and abroad

Method used

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  • Bacillus tubercle gene vaccine for treating medicine-tolerant pulmonary tuberculosis
  • Bacillus tubercle gene vaccine for treating medicine-tolerant pulmonary tuberculosis
  • Bacillus tubercle gene vaccine for treating medicine-tolerant pulmonary tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] It is used to prepare the Mycobacterium tuberculosis gene vaccine for treating drug-resistant tuberculosis, one of which is that the Mycobacterium tuberculosis Ag85A coding gene sequence is cloned into the eukaryotic expression vector pVAX1.

[0131] (1) Gene sequence:

[0132] The gene sequence of the Mycobacterium tuberculosis Ag85A nucleic acid vaccine for the treatment of drug-resistant tuberculosis is as follows:

[0133] 1T TTTCCCGGC CGGGCTTGCC GG TGGAGTAC CTGCAGGTGC CGTCGCCGTC GATGGGCCGT

[0134] 61 GACATCAAGG TCCAATTCCA AAGTGGTGGT GCCAACTCGC CCGCCCTGTA CCTGCTCGAC

[0135] 121 GGCCTGCGCG CGC AGGACGACTTCAGCGGC TGGGACATCA ACACCCCGGC GTTCGAGTGG

[0136] 182 TACGACCAGT CGGGCCTGTC GGTGGTCATG CCGGTGGGTG GCCAGTCAAG CTTCTACTCC

[0137] 242 ACTGGTACC AGCCCGCCTG CGGCAAGGCC GGTTGCCAGA CTTACAAGTG GGAGACCTTC

[0138]301 CTGACCAGCG AGCTGCCGGG GTGGCTGCAG GCCAACAGGC ACGTCAAGCC CACCGGAAGC

[0139] 361 GCCGTCGTCG GTCTTTCGAT GGCTGCTTCT TCGGCGCTGA CGCTGGCGAT CTATCACCCC

[014...

Embodiment 2

[0189]It is used to prepare tubercle bacillus gene vaccine for treating drug-resistant tuberculosis. The coding gene sequence of Mycobacterium tuberculosis Ag85A and ESAT6 is cloned into eukaryotic expression vector pVAX1 to form ESAT6-Ag85A chimeric gene vaccine.

[0190] (1) Gene sequence:

[0191] The gene sequence of the Mycobacterium tuberculosis Ag85A nucleic acid vaccine for the treatment of drug-resistant tuberculosis is as follows:

[0192] 1T TTTCCCGGC CGGGCTTGCC GG TGGAGTAC CTGCAGGTGC CGTCGCCGTC GATGGGCCGT

[0193] 61 GACATCAAGG TCCAATTCCA AAGTGGTGGT GCCAACTCGC CCGCCCTGTA CCTGCTCGAC

[0194] 121 GGCCTGCGCG CGCAGGACGA CTTCAGCGGC TGGGACATCA ACACCCCGGC GTTCGAGTGG

[0195] 183 TACGACCAGT CGGGCCTGTC GGTGGTCATG CCGGTGGGTG GCCAGTCAAG CTTCTACTCC

[0196] 243 ACTGGTACC AGCCCGCCTG CGGCAAGGCC GGTTGCCAGA CTTACAAGTG GGAGACCTTC

[0197] 301 CTGACCAGCG AGCTGCCGGG GTGGCTGCAG GCCAACAGG ACGTCAAGCC CACCGGAAGC

[0198] 361 GCCGTCGTCG GTCTTTCGAT GGCTGCTTCT TCGGCGCTGA CGCTGGCGAT CT...

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Abstract

The invention relates to a method for preparing a mycobecterium tuberculosis gene vaccine used for curing drug-resistant tuberculosis, and is characterized in that the mycobacterium tuberculosis gene vaccine against the drug-resistant tuberculosis clones a coding gene sequence of the mycobacterium tuberculosis Ag85A to the eukaryotic expression vector pVAX1, or clones coding gene sequences of the Ag85A and the ESAT6 together to the eukaryotic expression vector pVAX1 to form the ESAT6-Ag85A chimeric gene vaccine. Separated immunity or combined chemotherapy can obviously relieve pathology change in lungs of mice with drug-resistant tuberculosis and can obviously reduce bacteria numbers in lungs and spleens of little mice.

Description

technical field [0001] The invention relates to the preparation of tubercle bacillus gene vaccine for treating drug-resistant tuberculosis. Background technique [0002] So far, tuberculosis is still the number one "killer" of infectious diseases in the world. The main reason is that BCG vaccine cannot effectively prevent tuberculosis in adults. start to pick up. The World Health Organization (WHO) reports that there are currently 20 million tuberculosis patients in the world, 8-10 million new tuberculosis patients are added every year, and 2-3 million people die of tuberculosis every year. The tuberculosis epidemic and drug resistance in my country are quite serious, and the number of tuberculosis patients ranks second in the world, second only to India. The results of the fourth national tuberculosis epidemiological sampling survey in 2000 showed that 550 million people in my country were infected with tuberculosis, and there were 4.51 million tuberculosis patients, with...

Claims

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Application Information

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IPC IPC(8): A61K39/04A61P31/06
Inventor 吴雪琼梁艳李忠明刘庆良张俊仙王兰阳幼荣李宁余琦史迎昌朱琰
Owner 中国人民解放军总医院第二附属医院
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