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Method for extracting total effective parts in mixture of angelica and ligusticum wallichii rhizome by supercritical carbon dioxide gradient

An effective site, supercritical technology, applied in chemical instruments and methods, purification/separation/stabilization of organic compounds, organic chemical methods, etc.

Inactive Publication Date: 2011-06-08
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem of incomplete and low-purity extraction of the mixed medicinal materials of Angelica and Rhizoma Chuanxiong in the prior art, the present invention has carried out the comparison of combined extraction and fractional extraction After the study, the drug pair was mixed for supercritical CO2 extraction, and the three effective components of total phthalides, total organic acids and total alkaloids were sequentially extracted from the mixed medicinal materials of angelica and chuanxiong. parts, and taking the content and transfer rate of total phthalides, ligustilide, total organic acids, ferulic acid, total alkaloids, and ligustrazine as evaluation indicators, the optimal process for gradient extraction of the three effective parts was optimized condition

Method used

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  • Method for extracting total effective parts in mixture of angelica and ligusticum wallichii rhizome by supercritical carbon dioxide gradient
  • Method for extracting total effective parts in mixture of angelica and ligusticum wallichii rhizome by supercritical carbon dioxide gradient
  • Method for extracting total effective parts in mixture of angelica and ligusticum wallichii rhizome by supercritical carbon dioxide gradient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Put 200g of the mixed medicinal material (1:1) powder (40 mesh) of Angelica sinensis and Rhizoma Chuanxiong into the 1L extraction kettle of the supercritical extraction device, and use supercritical CO 2 Perform gradient extraction.

[0046] The medicinal materials were first subjected to the first gradient supercritical CO 2 Extract the total phthalide components, the extraction conditions are: the extraction temperature is 45°C, the extraction pressure is 25MPa, CO 2 The flow rate was 30 L / h, the extraction time was 2.0 h, the temperature and pressure of the separation tank I were 50 °C and 8 MPa, and the temperature and pressure of the separation tank II were 30 °C and 6 MPa, respectively.

[0047] The dregs extracted by the first gradient were taken for the second gradient supercritical CO 2 To extract the total organic acid components, the entrainer used is 300ml of 90% ethanol, and the extraction conditions are: the extraction temperature is 70°C, the extractio...

Embodiment 2

[0051] Put 200 g of the mixed medicinal material (1:2) powder (40 mesh) of Angelica and Rhizoma Chuanxiong into the 1 L extraction kettle of the supercritical extraction device, and use supercritical CO 2 Perform gradient extraction.

[0052] The medicinal materials were first subjected to the first gradient supercritical CO 2 Extract the total phthalide components, the extraction conditions are: the extraction temperature is 40°C, the extraction pressure is 30MPa, CO 2 The flow rate was 45 L / h, the extraction time was 1.5 h, the temperature and pressure of the separation tank I were 40 °C and 7.0 MPa, and the temperature and pressure of the separation tank II were 20 °C and 6.5 MPa, respectively.

[0053] The dregs extracted by the first gradient were taken for the second gradient supercritical CO 2 To extract the total organic acid components, the entrainer used is 200ml of 80% ethanol. The extraction conditions are: extraction kettle temperature 60°C, pressure 40MPa; sep...

Embodiment 3

[0057] Put 200 g of the mixed medicinal material (1:0.5) powder (40 mesh) of Angelica sinensis and Rhizoma Chuanxiong into a 1 L extraction kettle of a supercritical extraction device, and use supercritical CO 2 Perform gradient extraction.

[0058] The medicinal materials were first subjected to the first gradient supercritical CO 2 Extract the total phthalide components, the extraction conditions are: the extraction temperature is 50°C, the extraction pressure is 20MPa, CO 2 The flow rate was 20 L / h, the extraction time was 3.0 h, the temperature and pressure of the separation tank I were 30 °C and 6.0 MPa, and the temperature and pressure of the separation tank II were 40 °C and 5.5 MPa, respectively.

[0059] The dregs extracted by the first gradient were taken for the second gradient supercritical CO 2 The total organic acid components were extracted, and the entrainer used was 400ml of absolute ethanol. The extraction conditions are: extraction kettle temperature 75°C...

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Abstract

The present invention provides a supercritical CO2 gradient extraction method of total effective parts in the mixture of Chinese angelica and Szechuan lovage rhizome, the total effective parts are total phthalides, total organic acids and total alkaloids, the method comprises: a) the mixed herbal medicine powder of Chinese angelica and Szechuan lovage rhizome is poured into an extraction kettle of a supercritical extraction device; b) the components of the total phthalides in the herbal medicine is firstly extracted by the first gradient supercritical CO2; c) after the first gradient extraction, the components of the total organic acids of medicine residues is extracted by second gradient supercritical CO2; d) after the first gradient extraction and the second gradient extraction, the components of the total alkaloids in the medicine residues is extracted by the third gradient supercritical CO2; e) the supercritical extracts of the first, the second and the third gradient extractions are blended. The content of the total effective parts in the extract obtained by the method is 80-96 percent.

Description

technical field [0001] The invention relates to the field of traditional Chinese herbal medicine, in particular to supercritical CO 2 Gradient extraction method of total phthalides, total organic acids and total alkaloids in the mixture of angelica and chuanxiong. Background technique [0002] Angelica sinensis is the dried root of Angelica sinerisis (Oliv.) Diels of Umbelliferae. Angelica belongs to the Liver, Heart, and Spleen Meridians, and its medicinal properties are sweet, pungent, and warm. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and defecating. For blood deficiency and yellow complexion, dizziness and palpitations, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to deficiency and cold, constipation due to dryness of the intestine, rheumatic arthralgia, bruises, carbuncle sores. Angelica is a traditional Chinese medicine, which is widely...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/236C07B63/00
CPCY02P20/54
Inventor 杨义芳许玫
Owner SHANGHAI INST OF PHARMA IND CO LTD