Albumin based polyacrylic acid nano-carrier and preparation method thereof

A polyacrylic acid and nano-carrier technology, which is applied in the direction of inactive components of polymer compounds, can solve the problems of complex process, high equipment requirements, poor biocompatibility and biodegradability, etc., and achieves simple preparation process and strong hydrophilicity. , improve the effect of drug efficacy

Inactive Publication Date: 2009-04-08
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a simple and effective whitening method for the shortcomings of the prior art in the preparation of nano-microspheres and nano-capsules, such as complex process, high equipment requirements, use of organic solvents, poor biocompatibility and biodegradability, etc. Preparation method of protein-based polyacrylic acid nanocarrier

Method used

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  • Albumin based polyacrylic acid nano-carrier and preparation method thereof
  • Albumin based polyacrylic acid nano-carrier and preparation method thereof
  • Albumin based polyacrylic acid nano-carrier and preparation method thereof

Examples

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Effect test

Embodiment 1

[0039] Embodiment 1: (a) take a certain amount of bovine serum albumin solid, and make it into an albumin solution with a concentration of 0.005 mg / mL; (b) purify acrylic acid by vacuum distillation; (c) take the above-mentioned prepared albumin solution Put 10 mL of protein solution in a 50 mL three-necked flask, add 0.2 mg of purified acrylic acid, stir mechanically for 5 min to fully disperse acrylic acid in the albumin solution; (d) stir the mixed solution under nitrogen protection for 10 min, then heat up to 80°C, add 0.006 mg K 2 S 2 o 8 As an initiator, 0.004mg TEMED was used as an accelerator, and reacted for 4 hours under mechanical stirring; (f) after the reaction, the solution was cooled to room temperature, filtered, and the filtrate was dialyzed at room temperature for 45 hours to remove the initiator, unreacted acrylic acid and free acrylic acid oligomers to obtain albumin / polyacrylic acid nano-microspheres; (e) the dialyzed solution was allowed to stand at roo...

Embodiment 2

[0041] Example 2: (a) take a certain amount of bovine serum albumin solid, and make it into an albumin solution with a concentration of 0.005 mg / mL; (b) purify acrylic acid by vacuum distillation; (c) take the above-mentioned prepared albumin solution Put 10 mL of protein solution in a 50 mL three-necked flask, add 1.0 mg of purified acrylic acid, and stir mechanically for 10 min to fully disperse acrylic acid in the albumin solution; (d) Stir the mixed solution for 10 min under nitrogen protection, then heat up to 80°C, add 0.03 mg K 2 S 2 o 8 , 0.02g TEMED as an initiator, reacted for 4 hours under mechanical stirring; (f) after the reaction, the solution was cooled to room temperature, filtered, and the filtrate was dialyzed at room temperature for 45 hours to remove the initiator and unreacted acrylic acid and free acrylic acid oligomerization body to obtain albumin / polyacrylic acid nanospheres; (e) the dialyzed solution was allowed to stand at room temperature for 15 da...

Embodiment 3

[0043] Example 3: (a) take a certain amount of bovine serum albumin solid, and make it into an albumin solution with a concentration of 0.005 mg / mL; (b) purify acrylic acid by vacuum distillation; (c) take the above-mentioned prepared albumin solution Put 10 mL of protein solution in a 50 mL three-necked flask, add 1.0 mg of purified acrylic acid, and stir mechanically for 10 min to fully disperse acrylic acid in the albumin solution; (d) Stir the mixed solution for 10 min under nitrogen protection, then heat up to 65°C, add 0.03 mg K 2 S 2 o 8 , 0.02mg TEMED was used as an initiator, and reacted for 4 hours under mechanical stirring; (f) after the reaction, the solution was cooled to room temperature, filtered, and the filtrate was dialyzed at room temperature for 45 hours to remove the initiator, unreacted acrylic acid and free acrylic acid oligomerization body to obtain albumin / polyacrylic acid nanospheres; (e) the dialyzed solution was allowed to stand at room temperatur...

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Abstract

The invention provides a method for preparing an albumin-based polyacrylic acid nano-carrier, which uses biological macromolecule bovine serum albumin with strong hydrophilicity and good biocompatibility as a template, and makes use of a method in which acrylic acid in an albumin solution has self-assembly and further has in situ polymerization to successfully prepare nano-scale bovine serum albumin-based polyacrylic acid nanospheres and nanocapsules. The structures and the shapes of the prepared nanoshperes and the nanocapsules are characterized through a nanometer particle diameter instrument (DLS), an infrared absorption spectroscoper (FT-IR), UV-Vis spectrometer and transmission electron microscope (TEM) to find that the particle diameter of the nanoparticles is between 80 and 100 nanometers, the external diameter of the nanocapsules is between 300 and 400 nanometers, and the inner diameter thereof is about between 100 and 200 nanometers. The nanospheres and the nanocapsules are provided with positive charges on the surfaces and have pH sensitivity; the controllable release property of medicament doxorubicin is up to 150 hours; and the nanospheres and the nanocapsules are used for medicament carriers with strong toxicity and poor stability to achieve the controllable release of such medicaments so as to endow the medicaments with targeting property, improve the medicament efficacy, and reduce the toxicity and the side effects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the preparation of a drug carrier, in particular to a bovine serum albumin-based polyacrylic acid nano-microsphere and a nano-capsule drug carrier and a preparation method thereof. It is mainly used as a drug carrier with high toxic side effects and poor stability to realize the controlled release of such drugs, make the drugs targeted, improve drug efficacy, and reduce toxic and side effects. Background technique [0002] Albumin is a biopolymer composed of nearly 600 amino acid units. The amino acids are connected by peptide chains and twisted into earthworm or honeycomb shapes. There are numerous mesh-like gaps, which create favorable space conditions for mosaic-carrying drugs. Albumin has the advantages of safety, non-toxicity, non-immunogenicity, biodegradability, and good biocompatibility. Therefore, albumin is a multifunctional protein. It has many important physiological and ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42
Inventor 王荣民张慧芳何玉凤李刚
Owner NORTHWEST NORMAL UNIVERSITY
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