Antihuman Endoglin single-chain antibody, preparation and uses thereof

A single-chain antibody and carrier technology, which is applied in the fields of botany equipment and methods, biochemical equipment and methods, antibodies, etc., and can solve the problem of few research reports on anti-human Endoglin scFv

Inactive Publication Date: 2009-04-08
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] But so far, there are very few res

Method used

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  • Antihuman Endoglin single-chain antibody, preparation and uses thereof
  • Antihuman Endoglin single-chain antibody, preparation and uses thereof
  • Antihuman Endoglin single-chain antibody, preparation and uses thereof

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Embodiment Construction

[0035] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0036] 1. Construction of anti-human Endoglin scFv phage surface display library

[0037] 1. Animal immunity

[0038] Using the rhEndoglin extracellular segment protein (purchased from R&D Company) as an antigen, 6-week-old female healthy Balb / c mice (purchased from the Experimental Animal Center of the Third Military Medical University of the Chinese People's Liberation Army) with a body weight of 16-18 g were immunized. The immunization steps were as follows: For the first immunization, the an...

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PUM

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Abstract

The invention discloses an antihuman Endoglin single-chain antibody, with the amino acid sequence shown as SEQ ID No.2, also discloses coded genes of the antihuman Endoglin single-chain antibody, a recombination carrier containing the coded genes, a transformant containing the recombination carrier and a radionuclide maker of the antibody Endoglin single-chain antibody, as well as discloses a preparation method of the antihuman Endoglin single-chain antibody, including such two steps as solubility expression and purification. The method is simple and easy to be operated, the stability of product is good and the purity is high. The research result shows that the antihuman Endoglin scFv is characterized by small molecular weight, strong penetration, low heterology, fast removal in vivo, shortage of Fc segments and the like, has excellent affinity and stability of the antigen, can be used as a guiding carrier of target-direction tumor vessel endothelium, and is used for the preparation of tumor diagnosis reagents and immunization therapy medicaments, thus having promising application prospect.

Description

technical field [0001] The present invention relates to an antibody, in particular to an anti-human Endoglin single chain antibody (single chain variable fragment, scFv), and also to a preparation method and application of the scFv. Background technique [0002] Endoglin, also known as CD105, is one of the components of the transforming growth factor-β (TGF-β) superfamily receptor complex, regulates the response of cells to TGF-β, participates in angiogenesis, and is part of the endothelial cells at the edge of tumor tissue where blood vessels originate It is highly expressed on tumor angiogenesis and is closely related to tumor angiogenesis, and has become an important biological target molecule for tumor diagnosis, treatment, and inhibition of tumor angiogenesis. [0003] Compared with monoclonal antibodies, scFv has the characteristics of small molecular weight, strong penetrating power, low heterogeneity, fast clearance in vivo, lack of Fc segment, etc., and has good ant...

Claims

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Application Information

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IPC IPC(8): C07K16/28C12N15/13C12N15/63C12N1/21A61K51/00A61K39/395A61P35/00A61K103/10
Inventor 梁志清赵泽文
Owner ARMY MEDICAL UNIV
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