Fluorescence reader
A reader and fluorescence technology, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of collection efficiency, optical analysis performance, etc.
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[0075] According to a first embodiment, the fluorescence reader comprises a light source 5 , a detector 6 and a lens arrangement 17 arranged to focus the emitted fluorescence onto the detector 6 . The lens device 17 is provided with a total internal reflection suppressing member 16 comprising a layer of a suitable material with a suitable refractive index placed in physical contact with the substrate surface 3 . The material of this layer is preferably a soft polymer, such as silicon, epoxy, polyurethane (polyurethane) or acrylate, which is applied as an optically wetting layer on the surface of the lens device 17, with no contact between the layer and the surface. has air. Thus, light rays traveling to the lens do not pass through any air layer, which would result in a loss of reflection at higher angles. An additional advantage of choosing a soft polymer material is its ability to form an optically wetting layer on surfaces that are not perfectly uniform or flat. Furthermo...
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