Unlock instant, AI-driven research and patent intelligence for your innovation.

Accurate positioning method based on atomic force microscope

An atomic force microscope and precise positioning technology, which is applied to the parts of the instrument, instruments, surface/boundary effects, etc., can solve the problems of time-consuming consumables, substrate damage scales, and insufficient fast and convenient positioning, so as to avoid the problem of time-consuming consumables Effect

Inactive Publication Date: 2013-04-24
WUHAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For an atomic force microscope without a supporting optical system, it is difficult to precisely locate a specific position
The Chinese patent "Scanning Probe Microscope Sample Positioning Method" of Patent No. 99126338.3 reports a method, which is to divide a small area on one side of a transparent substrate and mark different numbers, foreign letters or various digital symbols and other identifiers to show difference, or after marking various identifiers in the small squares of the coordinate axis graph paper, paste them on the reverse side of the transparent substrate with double-sided tape or glue, or use etching technology to etch the coordinates on one side of the transparent substrate and lattice, the defect of this method is that it needs to be marked on the back of the substrate or use existing materials such as coordinate paper to mark, resulting in damage to the substrate or blurring of the scale, and the positioning is not fast and convenient enough. In addition, it is not very good. The influence of the tip shape of the atomic force microscope on the measurement is completely ruled out
The method provided by Chinese Patent Application No. 200410084487.2 "Repositioning Method Based on Atomic Force Microscope" requires self-made positioning tools, and this method is to process the substrate. Since the substrate is a consumable, it is time-consuming and consumable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Accurate positioning method based on atomic force microscope
  • Accurate positioning method based on atomic force microscope
  • Accurate positioning method based on atomic force microscope

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Observation of maize metaphase chromosomes by atomic force microscope.

[0020] 1. Conventional chromosome preparation method Maize metaphase chromosomes were prepared on glass slides.

[0021] 2. Use an optical phase contrast microscope to find the chromosome to be tested.

[0022] 3. Under the optical microscope, the copper sheet PELCO Position the Center-Marked Grids above the sample to be tested, record the area where the sample is located, and use the adsorption force to place the PELCO Center-Marked Grids are sucked onto the substrate of the sample.

[0023] 4. Attach the PELCO The base of Center-Marked Grids is attached to the stage of atomic force microscope with double-sided tape.

[0024] 5. Scanning PELCO with AFM Center-Marked Grids, the scanning range is reduced step by step, and the probe of the atomic force microscope is positioned above the corresponding grid of the sample to be tested.

[0025] 6. Use the ear wash ball to put PELCO Center-Ma...

Embodiment 2

[0027] Comparison of Maize Metaphase Chromosomes Before and After DNase I Treatment Using Atomic Force Microscopy

[0028] 1. Conventional chromosome preparation method Maize metaphase chromosomes were prepared on glass slides.

[0029] 2. Use an optical phase contrast microscope to find the chromosome to be tested.

[0030] 3. Under the optical microscope, the copper mesh PELCO Position the CenterMarked Grids above the sample to be tested, record the area where the sample is located, and use the adsorption force to place the PELCO CenterMarked Grids are sucked onto the substrate of the sample.

[0031] 4. Attach the PELCO The base of Center-Marked Grids is attached to the stage of atomic force microscope with double-sided tape.

[0032] 5. Scanning PELCO with AFM Center-Marked Grids, the scanning range is reduced step by step, and the probe of the atomic force microscope is positioned above the corresponding grid of the sample to be tested.

[0033] 6. Use the ear w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for precise orientation by an atomic force microscope. The precise orientation method comprises steps of: firstly finding a sample to be measured by an optical microscope; putting a copper net Center_Marked Grids above the sample to be measured, the copper net being formed by ruled grids with the same size, recording the region of the grids corresponding to the sample to be measured under the optical microscope, scanning the Center_Marked Grids by using an atomic force microscope, reducing the scanning area stage by stage, locating a probe of the atomic force microscope above the corresponding grids of the sample to be measured; and finally, softly blowing the Center_Marked Grids from side by using a washing ear ball; thereby carrying out the precise observation of the atomic force microscope by knitting stitch. The method can precisely orientate the sample to be scanned.

Description

technical field [0001] The invention relates to an accurate positioning method based on an atomic force microscope, that is, a method for accurately positioning a sample in a micro region by using an atomic force microscope. technical background [0002] AFM is an important tool for studying the surface structure of objects at the nanometer scale, and has been widely used in physics, biology, chemistry and other research. At the beginning of the invention, the atomic force microscope has been used for physical research. The universal uniformity of physical materials determines that it is not necessary to image a specific sample, so that it is not necessary to accurately position the sample to be tested. Therefore, some manufacturers' atomic force microscopes do not have supporting optical systems ( Such as the 9500J3 atomic force microscope of Shimadzu Corporation of Japan, and the Dimension 3100 atomic force microscope of Veeco Precision Instrument Co., Ltd. of the United S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N13/16G12B21/20G01Q10/00
CPCG01Q40/00
Inventor 李立家马璐
Owner WUHAN UNIV