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Use of oligosaccharide in preparing medicine for treating tumor and inhibiting tumor metastasis

A technology of tumor metastasis and chitosan oligosaccharide, which is applied in the application field of medicine or health food, to achieve the effect of anti-tumor and inhibition of tumor metastasis, inhibition of tumor angiogenesis, and efficient tumor metastasis

Inactive Publication Date: 2009-04-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, tumor metastasis is a serious challenge to cancer therapy

Method used

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  • Use of oligosaccharide in preparing medicine for treating tumor and inhibiting tumor metastasis
  • Use of oligosaccharide in preparing medicine for treating tumor and inhibiting tumor metastasis
  • Use of oligosaccharide in preparing medicine for treating tumor and inhibiting tumor metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Migration experiment

[0027] Human umbilical vein endothelial cells were divided into 0.1-10×10 6 pcs / ml (in this example, 1-2×10 6 Cells / ml) were inoculated in a 6-well plate, cultured for 10-24h (24h in this example), after the human umbilical vein endothelial cells grew into a single layer in a 6-well culture plate, scratched with a cell scraper, and washed with PBS (120mM Nacl, 5mMKcl, 1.7mMKH 2 PO 4 , 8.3mM NaHPO 4 .12H 2 O) rinse 3 times, then add the chitosan oligosaccharides with a degree of polymerization of 2-8 into the culture medium, the final concentrations are respectively 5ug / ml, 50ug / ml and 500ug / ml, and simultaneously use the tumor cell culture medium as an inducing factor, Continue culturing for 10-24h (24h in this example) and observe the results under a phase-contrast microscope.

[0028] Depend on figure 1 It can be seen that the tumor cell culture medium can significantly promote the migration of human umbilical vein endothelia...

Embodiment 2

[0030] Example 2 Forming a tubular structure experiment

[0031] Use 10-100ul (50-100ul in this example) mouse tail collagen to pre-spread a 96-well plate, and after solidification, use 0.1-10×10 5 pcs / ml (in this example, 1-2×10 5 Human umbilical vein endothelial cells were inoculated at a density of 1 / ml), cultured for 10-24 hours (24 hours in this example), and then oligochitosan with a degree of polymerization of 2-8 was added to the culture medium with a final concentration of 50ug / ml. The cell culture medium is an inducing factor, and the results were observed under a phase-contrast microscope after continuing to culture for 10-48 hours (24 hours in this example).

[0032] Depend on image 3 It can be seen that chitosan oligosaccharides can inhibit the induction of tumor cell culture medium on the formation of tubular structures of human umbilical vein endothelial cells.

Embodiment 3

[0034] Human umbilical vein endothelial cells were divided into 0.1-10×10 6 pcs / ml (in this example, 1-2×10 6 cells / ml) in a 96-well plate, and after culturing for 10-24 hours, add the drug. At the same time, use the tumor cell culture medium as an induction factor, continue culturing for 10-24 hours (24 hours in this example), and then add MTT (Blue Tetrazolium) ) reagent and DMSO, and after 1-5h (this example adopts 3h), use a microplate reader to detect the inhibitory effect of the drug on cell proliferation.

[0035]Depend on Figure 4 It can be seen that chitosan oligosaccharides can inhibit the promotion of tumor cell culture medium to the growth of umbilical vein endothelial cells in the concentration range of 5-50ug / ml, and it is positively correlated with the concentration, but this inhibitory effect is not significant after the concentration exceeds 200ug / ml. obvious.

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Abstract

The invention relates to the biological medicine, in particular to the application of chitosan oligosaccharide in preparing medicines used for treating tumors and restraining tumor metastasis, wherein, by taking a nutrient solution for tumor cells as an inducing factor and taking a human umbilical vein endothelial cell as an study object, the chitosan oligosaccharide can significantly restrain the human umbilical vein endothelial cell metastasis induced by the nutrient solution for tumor cells and the formation of tubular structure caused by the endothelial cell metastasis, but have no obvious restraining effect on the proliferation of the human umbilical vein endothelial cells metastasis induced by the nutrient solution for tumor cells.

Description

technical field [0001] The medicine for treating tumor of the present invention is specifically the application of chitosan oligosaccharide in the preparation of medicine or health food for treating tumor and inhibiting tumor metastasis. Background technique [0002] With the development of human society, the aggravation of environmental pollution, and the change of lifestyle, tumors, especially malignant tumors-cancer, have become one of the major diseases that threaten human life. According to statistics, malignant tumors have become the first or second cause of human death, and about 7 million people die of cancer in the world every year. Although compared with some developed countries, such as the United States, the United Kingdom, France, Russia, Japan, etc., the mortality rate of malignant tumors in my country and the proportion of total deaths are relatively low, but malignant tumors are also ranked first among various causes of death nationwide. The second place, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/722A61P35/00A61P35/04
Inventor 杜昱光吴海歌白雪芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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