Method for breeding cuttage poplar seedlings

A technology for cutting seedlings and poplar trees, which is applied in the field of cutting poplar seedling breeding, which can solve problems such as poor tree growth, ecological damage, and poor site conditions, so as to improve the ability to resist adverse environmental influences, promote root growth, and increase survival rate Effect

Inactive Publication Date: 2009-05-20
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

Because the geographical location of poplar planting in my country mainly depends on human factors, it is different from the growth of poplar in nature, resulting in a single tree species in some areas, poor site conditions, coupled with poor management, resulting in large-scale disease and poor tree growth. , or even die, eventually causing huge economic losses and ecological damage to poplar planting areas

Method used

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  • Method for breeding cuttage poplar seedlings

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Effect test

Embodiment 1

[0015] A breeding method for cutting poplar saplings, comprising the following steps: preparation of a solid bacterial agent: taking mycorrhizal fungal strains and using a potato juice medium to activate them on a slant, and then inserting the activated bacterial strains into a culture medium containing potato juice Cultivate in a petri dish until the whole petri dish is covered, and then transfer the mycorrhizal fungi in the petri dish to a conical flask containing potato juice culture solution for dark cultivation on a shaking table, with the shaking table speed set at 110-121 / min The time is 5-7 days. After a large number of mycelium balls are formed in the Erlenmeyer flask, the mycorrhizal fungi are inserted into the solid culture medium, and cultivated in the dark at a constant temperature of 25°C-28°C. After the mycelium grows from the top of the medium to the bottom Take it out for later use; the solid microbial culture medium is: vermiculite, peat, river sand, cottonsee...

Embodiment 2

[0017] Follow the steps below

[0018] 1. Preparation of poplar cuttings: annual 895 poplars, the length of each cutting is 15cm, and the upper band can germinate 3. The cutting time in Jiangsu Province is from the end of March to the first ten days of April. The cuttings are cut one day before cutting and soaked in water for 24 hours.

[0019] 2. Mycorrhizal agent preparation: the preserved bad-tasting milk mushroom (Lactarius insulsus) was used as a strain (purchased from Northeast Forestry University) to activate the slant with potato juice medium, and then inserted into the culture medium containing potato juice culture medium. Culture in the dish until the whole Petri dish is covered, then transfer the mycorrhizal fungi in the Petri dish into a 100ml Erlenmeyer flask filled with 40ml of potato juice culture solution for dark culture on a shaking table, the speed of the shaking table is set to 121 / min, and the culture The time is 5-7 days. After a large number of mycelium...

Embodiment 3

[0022] Embodiment 3: carry out according to the following steps

[0023] 1. Preparation of poplar cuttings: 72 poplars per year, each cutting is 15cm long, and 3 buds can sprout on it. The cutting time in Jiangsu Province is from the end of March to the first ten days of April. The cuttings are cut one day before cutting and soaked in water for 24 hours.

[0024] 2. Mycorrhizal agent preparation: the preserved colored bean puffball (Pisolithus tinctorius) was used as a strain (obtained from Nanjing Forestry University) to activate the slant with potato juice medium, and then inserted into a petri dish containing potato juice medium Cultivate in medium until the whole petri dish is covered, then transfer the mycorrhizal fungus in the petri dish into a 100ml Erlenmeyer flask filled with 40ml potato juice culture solution for dark cultivation on a shaking table, the shaking table speed is set to 121 / min, and the cultivation time For 5-7 days, a large number of mycelium balls are...

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Abstract

The invention provides a breeding method for poplar seedling stem planting, which comprises the following steps: the preparation of a solid fungus agent; the treatment of poplar stems; mycorhiza inoculation; and the management of mycorhiza seedlings. The breeding method can improve the resistance of the poplar stem planting seedlings and promote the growth of root systems, thereby improving the survival rate of poplar, the resistance of poplar to adverse environments and the accumulated amount of wood.

Description

1. Technical field [0001] The invention belongs to the technical field of sapling breeding in modern agricultural technology, and in particular relates to a cutting poplar sapling breeding method. 2. Background technology [0002] Prior art: Populus (Populus) is an excellent fast-growing timber tree species, which is widely distributed in the northern hemisphere, and there are more than 50 tree species in China alone. Because poplar grows rapidly, asexual reproduction is simple and easy to survive, it is widely planted in my country and is the main economically cultivated tree species in Jiangsu Province. Only 400 million poplars are planted in northern Jiangsu, and the output of poplar processing industry in Jiangsu ranks first in the country . Poplar not only provides boards, but also is the main tree species for windbreak and sand fixation. Mycorrhizae are a combination of mycorrhizal fungi in soil and the vegetative roots of higher plants. Mycorrhizal fungi can absorb ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00C12N1/14
Inventor 宋微吴小芹叶建仁刘进华
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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