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Effective component of cowherb seed as well as preparation method and use thereof

A technology of Wang Bu Liuxing and effective components, which is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve the problems of normal cells and side effects.

Inactive Publication Date: 2012-04-18
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of cowherb seed as well as preparation method and use thereof
  • Effective component of cowherb seed as well as preparation method and use thereof
  • Effective component of cowherb seed as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] The preparation of embodiment 1 Wangbuliuxing effective component

[0085] 250g of Wangbuliuxing was pulverized, added 8 times the amount of ethyl acetate: ethanol = 1:1, heated and refluxed for 1 hour, extracted twice, added 8 times the amount of 70% ethanol to the dregs, heated and refluxed for 1 hour, extracted twice, and the filtrate Merge the extracts, concentrate the extracts, dissolve the sample with 5% ethanol, and pass through the ODS-C18 column. First, use 5% ethanol as the mobile phase, and then change 50% ethanol as the mobile phase to obtain the eluent. Preparative liquid chromatography continues to separate the eluent, separation conditions: the chromatographic column is an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0086] Time (min)

[0087] 54

[0088] The flow rate is 10ml / min, and the column temperature is room tempe...

Embodiment 2

[0089] The analysis of embodiment 2 Wangbuliuxing effective components

[0090] The HPLC-ELSD joint analysis to the effective component of the wangbuliuxin of embodiment 1

[0091] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 70% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 30% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 50% 0.2% glacial acetic acid aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% ...

Embodiment 3

[0094] Embodiment 3 Wangbuliuxing effective component preparation

[0095] Take any one of the effective components of Wangbuliuxing in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, and move to the drip irrigation after the material is changed, and the medicine liquid drops to 6-8 ℃ in liquid paraffin, degreasing, made 400 dropping pills.

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Abstract

The invention discloses an effective composition of saponaria vaccaria. The preparation process comprises the following: step one: a mixture of acetic ether and ethanol is used as a solvent and carries out extraction on saponaria vaccaria; step two: medicine residue is extracted through ethanol to obtain an extraction solution; step three: the extraction solution is subjected to column chromatography to obtain an eluant; and step four: the eluant prepared through liquid phase chromatographic gradient elution uses water and acetonitrile as a mobile phase; and the eluant is collected for 36.0 to 40.0 minutes to obtain the effective composition. The invention also provides a method for preparing the effective composition, a pharmaceutically acceptable preparation and application of the effective composition to preparing an antitumor drug.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from wangbuliuxing, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/36A61P35/00A61K131/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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