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Human influenza-poultry influenza combined vaccine and preparation method thereof

A combined vaccine and influenza vaccine technology, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, antibody medical components, etc., can solve the problems of difficulty in avian influenza vaccination and low cost, and achieve the solution of difficulty in vaccination and low production procedures , high safety effect

Inactive Publication Date: 2009-06-10
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a combined human influenza-avian influenza vaccine, adding human avian influenza vaccine to the human influenza vaccine, which has low cost and is easily accepted by people, and solves the problem of difficulty in inoculating the avian influenza vaccine

Method used

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  • Human influenza-poultry influenza combined vaccine and preparation method thereof
  • Human influenza-poultry influenza combined vaccine and preparation method thereof
  • Human influenza-poultry influenza combined vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Access to combination vaccines:

[0049] 1.1 Chicken embryos for production

[0050] Chicken embryos used for passage and preparation of virus seeds should come from SPF chickens; chicken embryos used for vaccine production should come from healthy chickens raised in closed houses.

[0051] 1.2 Poison Seeds

[0052] 1.2.1 Name and source

[0053] Avian influenza strain: NIBRG-14 strain, derived from the National Institute for Biological Products Control (NIBSC); transformed from A / VietNam / 1194 / 2004 H5N1 strain by reverse genetic method, referred to as R1194 strain.

[0054] Influenza strains: strains recommended by WHO for the production of influenza vaccines in 2007-2008 - A1 / IVR-145, A3 / NYMC X-161B, B / Malysia-2506-2004.

[0055] 1.2.2 Establishment of seed batches

[0056] It should meet the requirements of the current version of the "Chinese Pharmacopoeia" three general rules "Management Regulations for Bacteria and Toxins Used in the Production and Testing of Bi...

Embodiment 2

[0172] 1. Experimental materials and procedures:

[0173] 1. Vaccine immunization animal experiment

[0174] The combined vaccine prepared by the method of Example 1 proves through various animal immunization tests that the sensitivity of chickens and mice is very poor, and rats are too sensitive, while rabbits are more ideal, showing that the antibody response is relatively stable and the amount- The effect is obvious, the intensity is moderate and close to that of the human body, the blood volume is large, and blood collection is easy.

[0175] The rabbits used in the experiment are New Zealand species, about 2 kg, male and female, from Zhejiang Academy of Agricultural Sciences.

[0176] 2. Vaccine immunization experiment in rabbits

[0177] 1) Dosage and grouping (each monovalent vaccine stock solution prepared by the method in Example 1 is diluted with 0.01mol / L PBS (pH7.2) to prepare a monovalent or multivalent vaccine with the following composition, and the adjuvanted ...

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Abstract

The invention provides a quadrivalence combined vaccine comprising H1N1, H3N2, B types flu and H5N1 type avian influenza and a preparing method. Concentration of each vaccine hemagglutinin is calculated with hemagglutinin: H1N1 type flu vaccine 20-40 mcg / mL, H3N2 type flu vaccine 20-40 mcg / mL, B type flu vaccine 20-40 mcg / mL and H5N1 type flu avian influenza 20-40 mcg / mL. The vaccine combining with human flu and avian influenza for human can solves problem of difficult to vaccination avian influenza vaccine, prevents avian influenza at the same time prevents huamn flu. Four indications (egg albumen, endotoxin, total protein contents, rate of total protein and hemagglutinin) The combined vaccine closly-related with side effect in the combined vaccine has low flu preparing regulation than national regulation, and has higher safety.

Description

(1) Technical field [0001] The invention relates to a human influenza-avian influenza combined vaccine and a preparation method thereof, in particular to a combined vaccine comprising H1N1 type, H3N2 type, B type influenza and H5N1 type avian influenza vaccine and a preparation method thereof. (2) Background technology [0002] On October 14, 2005, WHO appealed to the world: "Currently the whole world is on the brink of another influenza pandemic. Once it occurs, it will spread rapidly and all countries will be infected in less than 3 months. The underestimated death toll is between 2 million and 7.4 million, the economic and social devastation is enormous, and every country in the world must be prepared." [0003] From 2003 to September 10, 2008, a total of 387 H5N1 bird flu cases occurred around the world, 245 died, and the case fatality rate was as high as 63.3%. The development of the technology has attracted great attention from the governments of various countries. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/145A61P31/16
Inventor 朱智勇
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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