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Method for cultivating chlorella by heterotrophic nutrition fermentation

A technology of fermentation culture and chlorella, applied in the biological field, can solve problems such as high algal biomass, and achieve the effect of important application prospects and development value

Inactive Publication Date: 2009-07-15
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report of obtaining such a high algae biomass in such a short period of time at home and abroad.

Method used

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  • Method for cultivating chlorella by heterotrophic nutrition fermentation
  • Method for cultivating chlorella by heterotrophic nutrition fermentation
  • Method for cultivating chlorella by heterotrophic nutrition fermentation

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Embodiment Construction

[0013] 1. Chlorella medium: prepare 20 liters of medium, composition (1.0L deionized water): KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O 2.0g, NaCl 1.0g, NaHCO 3 0.2g, CaCl 2 10.0mg, FeSO 4 5.0mg, ZnCl 2 5.0 mg, MnCl 2 4H 2 O 5.0mg, CuCl 2 1.0mg. If the one-time addition of glucose and potassium nitrate is adopted, 78g / L glucose and 11.3g / L potassium nitrate (mass ratio of carbon and nitrogen is 20:1) are added to the culture medium; In another 5-liter glass bottle, prepare 2 liters of feed solution, which is composed of (1.0L deionized water): 800g glucose, KNO 3 115g (the mass ratio of carbon and nitrogen is 20:1). Both the culture medium and feed liquid were sterilized at 121° C. and 0.11 MPa high pressure for 20 minutes. After the temperature of the medium was lowered to 30° C., Chlorella species were inoculated for heterotrophic fermentation.

[0014] 2. Heterotrophic fermentation culture conditions of Chlorella: 50 liters of fully automatic fermentation tanks were ...

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Abstract

A method of heterotrophic culture of chlorella in fermentor belongs to the biotechnology field. The method adopts chlorella USTB-01 (culture collection number: CGMCC No. 1448, and collection date: Aug. 25, 2005) which is obtained after being screened. In the process of heterotrophic culture of chlorella USTB-01 in fermentor, the flow amounts of glucose and potassium nitrate are increasingly raised at three stages according to the different growing periods of chlorella USTB-01, so as to ensure the quick growth of chlorella USTB-01. After 2 days of culture, over 45g / L of chlorella biomass can be obtained. Therefore, the method lays a foundation for the efficient industrial heterotrophic culture of the chlorella in fermentor.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for cultivating Chlorella-USTB01 (Chlorella-USTB01) (CGMCC No. 1448, date of preservation: August 25, 2005) through efficient heterotrophic fermentation. Background technique [0002] There are about 10 species of chlorella in the genus Chlorella, all of which are rich in protein, unsaturated fatty acids, carotenoids, vitamins and chlorella factors, etc. function, has been listed as the health food of human beings in the 21st century. With the improvement of people's living standards, the demand for chlorella will increase. [0003] It is generally believed that chlorella belongs to lower plants, mainly absorbing inorganic carbon and synthesizing organic matter through photosynthesis. However, when the photoautotrophic culture method is adopted, when the concentration of algae cells increases to a certain number, the light must be blocked from entering the cultur...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 闫海景建克许倩倩刘硕杨晓静杨帅贾璇王子敬
Owner UNIV OF SCI & TECH BEIJING
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