Fast detection method for dihydro flavanol 4-reductase/leucocyanidin reductase in tree plant

A technology of cyanidin reductase and dihydroflavan, applied in the field of rapid detection of dihydroflavanol 4-reductase/leucocyanidin reductase in tea tree, can solve the problem of high cost, long detection time, enzyme The active method is cumbersome and other problems, and the effect of low cost, high accuracy and simple method is achieved
CN101482500AInactive Publication Date: 2009-07-15ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Publication Date
2009-07-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a quick detection method of dihydrogen-flavanol4-reductase / leucocyanidin reductase and anthocyanin reductase in tea tree, which can solve the problems of the prior technique of complicated operation in enzymatic activity detection method, high cost, long detection time. The quick detection method comprises: preparing an enzyme extracting-solution, measuring the protein content in enzyme extracting-solution and enzymatic activity, wherein the enzymatic activity is detected by detecting the consumption of the reduction type nicotinamide adenine dinucleotide phosphate solution (NADPH) and the vitamine C is added into the reaction system to reduce the interference of the substrate DHM and CYA on enzymatic activity detection. The quick detection method using ultraviolet spectrophotometer has features of simple method, low cost, short detection time of 15-25 minutes; environmental protection, safety, no need of noxious organic solvent, higher detection accuracy due to continuous detection of the changed enzymatic activity.
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Description

technical field

[0001] The invention relates to a detection method of dihydroflavanol 4-reductase / leucocyanidin reductase (DFR / LAR) and anthocyanin reductase (ANR) in tea tree. Background technique

[0002] Tea (Camellia sinensis) leaves are rich in flavonoids, alkaloids and other important secondary metabolites, which have important physiological and health effects on the human body, such as anti-allergic, anti-tumor and anti-aging, etc. [1] , is a popular health drink in the world.

[0003] Dihydroflavanol 4-reductase / leucocyanidin reductase (DFR / LAR) and anthocyanin reductase (ANR) are important enzymes in the downstream pathway of flavonoid synthesis and are also non-ester-type catechins. It is a direct enzyme of catechin synthesis and plays an important regulatory role in the tea tree catechin pathway.

[0004] At present, the determination methods of dihydroflavanol 4-reductase / leucocyanidin reductase (DFR / LAR) and anthocyanin reductase (ANR) enzymes at home and abro...

Claims

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