Fast screening method for mycobacterium tuberculosis
A rapid technology for Mycobacterium tuberculosis, applied in the field of medical detection, can solve the problems of low sensitivity, difficult to carry out, missed detection, etc., to achieve the effect of simple detection process, guarantee timely treatment, and increase the positive rate
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[0017] The rapid screening method of Mycobacterium tuberculosis of the present invention comprises the following steps:
[0018] Step 1: Specimen Liquefaction
[0019] Take the patient's sputum sample and mix it with a mixture of 2% NaOH and 0.5% N-acetyl-L-cysteine (liquefaction treatment reagent) in a ratio of 1:3 for about 10 minutes to completely liquefy the sample;
[0020] Step 2: Enrichment and purification of samples
[0021] Take polyclonal antibodies such as anti-LAM, anti-mpt64 or anti-TBGL, which are specific antibodies against Mycobacterium tuberculosis, and coat them on magnetic microbeads to form nano-immune magnetic beads coated with mouse anti-Mycobacterium tuberculosis specific membrane antibodies, and then Add 10-100ug of nano-immune magnetic beads to the patient specimen that has been completely liquefied in the first step, and stir fully to make them completely combined on the nano-immune magnetic beads; collect the above-mentioned nano-immune magnetic ...
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