Fast screening method for mycobacterium tuberculosis

Abstract

The invention discloses a method for fast screening Mycobacterium tuberculosis, comprising the following steps: firstly, liquefying specimens: adding liquefaction processing reagents in sputum specimens of patients and fully liquefying the sputum specimens; secondly, enriching and purifying specimens: coating specific antibodies of the Mycobacterium tuberculosis on a magnetic microballon, then adding the specific antibodies of the Mycobacterium tuberculosis in the liquefied specimens of patients, fully stirring the mixture to cause the mixture fully to be combined on an immune magnetic microballon and obtaining the well processed magnetic microballon to be tested after the washing of washing liquor; thirdly, foaming test: diluting and uniformly mixing the magnetic microballon to be tested using phosphate buffer solution to carry out the foaming test; if the specimens do not generate foams at room temperature, the specimens are negative tuberculosis; if the specimens generate foams at room temperature, and generate foams after being processed for 20 minutes at 68 DEG C after the foaming test, the specimens are negative; and if the specimens do not generate foams, the specimens are positive Mycobacterium tuberculosis. The whole detection process is simple, easily-operated, and highly precise and ensures the timely treatment of tuberculosis patients.

Description

technical field [0001] The invention relates to a medical detection method, in particular to a rapid screening method for mycobacterium tuberculosis which replaces conventional microscopic examination. Background technique [0002] As an ancient infectious disease, tuberculosis is on the rise globally. According to WTO estimates, about 2 billion people in the world are infected with Mycobacterium tuberculosis, with 8 million new cases and 3 million deaths every year. In my country, according to the fourth national tuberculosis survey in 2000, there were about 550 million tuberculosis cases in the country, nearly half of the country's population. The current situation of tuberculosis in our country is severe. It is an urgent task to establish advanced diagnostic techniques and treatment measures as soon as possible. It is not only related to the physical and mental health of tens of millions of people, but also an important issue related to national livelihood and national e...

AI Technical Summary

Problems solved by technology

Acid-fast staining is the current standard method for testing pulmonary tuberculosis, but the sensitivity of this method is low, and it is often missed due to human factors; the specificity of chest X-ray is not strong; although PCR has high sensitivity and specificity, it requires more operators and the environment. It is relatively high, and it is difficult for general primary medical institutions to carry out; ELISA is a new method that has only been developed in recent years, but there is no recognized good marker at present, resulting in the existence of one or another in the kits of various manufacturers. Disadvantages: Although the culture method is currently recognized as the gold standard for detecting Mycobacterium tuberculosis, it takes one month due to the slow growth of Mycobacterium tuberculosis, which seriously affects the timely treatment of patients