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Method for extracting Chinese actinidia root extract and application thereof for resisting liver damage

A technology of kiwi fruit root and extraction method, which can be applied to medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, etc., and can solve problems such as few studies

Inactive Publication Date: 2009-08-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the anti-hepatic damage of kiwi root in China, and there are few related studies abroad

Method used

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  • Method for extracting Chinese actinidia root extract and application thereof for resisting liver damage
  • Method for extracting Chinese actinidia root extract and application thereof for resisting liver damage
  • Method for extracting Chinese actinidia root extract and application thereof for resisting liver damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Put 1 kg of kiwi fruit root into a 10-liter conical flask, extract with 6 liters of 70% ethanol, stir for 3-4 hours, and extract three times at an extraction temperature of 80°C. Centrifuge to remove the residue, after concentration, go to macroporous resin (type AB-8 or X-5) chromatography column (60 × 600mm), use 50%, 60%, 70%, 80% ethanol gradient elution respectively, collect 60 -70% ethanol eluent. Concentrate the collected kiwifruit root eluate until ethanol-free to obtain a flocculent precipitate. After centrifugation, place the filter cake in a vacuum drying oven at a controlled temperature of 50-60°C for 3 hours. Get kiwi root extract.

Embodiment 2

[0046] Put 10kg of kiwifruit roots into a 100-liter extraction tank, extract with 60 liters of 80% ethanol, stir for 3-4 hours, and extract three times at an extraction temperature of 70°C. Centrifuge to remove residue, after concentration, go to macroporous resin (model AB-8 or X-5) chromatography column (100 × 1500mm), use 50%, 60%, 70%, 80% ethanol gradient elution respectively, collect 60 %, 70% ethanol eluent. Concentrate the collected kiwifruit root eluate until ethanol-free to obtain a flocculent precipitate. After centrifugation, place the filter cake in a vacuum drying oven at a controlled temperature of 50-60°C for 3 hours. Get kiwi root extract.

Embodiment 3

[0048] Put 50kg of kiwifruit roots into a 500-liter extraction tank, extract with 300 liters of 90% ethanol, stir for 3-4 hours, and extract three times at an extraction temperature of 60°C. Centrifuge to remove residue, after concentration, go to macroporous resin (model AB-8, X-5) chromatography column (300 × 1800mm), use 50%, 60%, 70%, 80% ethanol gradient elution respectively, collect 60 -70% ethanol eluent.

[0049] Concentrate the collected kiwifruit root eluate until ethanol-free to obtain a flocculent precipitate. After centrifugation, place the filter cake in a vacuum drying oven at a controlled temperature of 50-60°C for 3 hours. Get kiwi root extract.

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Abstract

The invention relates to an extraction method of a kiwi fruit root extract and an anti-liver injury application thereof, belonging to the fields of preparation and application technology of the kiwi fruit root extract. The invention takes kiwi fruit root or root bark as raw material, utilizes ethanol solution for extraction, further separates the extract on a column and extracts to obtain the kiwi fruit root extract containing active ingredients with anti-liver injury effect. The kiwi fruit root extract can significantly reduce alanine aminotransferase ALT in plasma of mice with acute carbon tetrachloride-induced liver injury, the decreasing range achieves 64.72 percent, which is greater than the effect of ammonium glycyrrhizinate, the kiwi fruit root extract can also significantly reduce serum aspartate aminotransferase AST, the decreasing range achieves 28.26 percent, which is not as good as the effect of the ammonium glycyrrhizinate. The invention provides a new valuable use for comprehensively utilizing the kiwi fruit root.

Description

technical field [0001] An extraction method of kiwifruit root extract and its anti-liver damage application, the invention relates to a method for extracting and separating active ingredients of kiwifruit root for anti-liver damage. The invention belongs to the technical field of preparation and application of kiwifruit root extract. Background technique [0002] Kiwifruit root is the root or root bark of Actinidia chinensis planch, which has the functions of clearing away heat, diuresis, promoting blood circulation, reducing swelling, curing hepatitis and anticancer. Effect. [0003] The research on the anti-hepatic damage of kiwi root has not been reported in China, and there are few related research abroad. [0004] When we studied the ethanol extracts from the roots of different species of kiwifruit, we found that they not only have good anti-tumor activity, but also have good anti-liver damage activity. [0005] In order to make full use of kiwifruit resources and pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P1/16C07J63/00A61K125/00
Inventor 徐德平裘爱泳唐年初刘国华来燕
Owner JIANGNAN UNIV
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