Method for improving Fraxinus mandshurica somatic embryo development synchronization

Abstract

The invention discloses a method for improving the synchronization of the development of the somatic embryos of fraxinus mandshurica, which relates to a method for improving the synchronization of the development of somatic embryos. The method solves the problems of the prior technology for the development of the somatic embryos of the fraxinus mandshurica, such as asynchronous development of the somatic embryos, low survival rate of somatic embryo regeneration plants caused by low somatic embryo development consistency, and high somatic embryo abnormal rate caused by chemical regulation. The method comprises the following steps that: I) explants are inoculated in a somatic embryo inductive culture medium to be cultured to form spherical somatic embryos; II) the spherical somatic embryos are inoculated in a synchronization regulating culture medium to be cultured to form mature somatic embryos; and III) the mature somatic embryos are inoculated in a germination culture medium after being dried to be cultured to form fraxinus mandshurica plantlets, and thus the improvement in the synchronization of the development of the somatic embryos of the fraxinus mandshurica is completed. The method realizes the physical regulation of the synchronous development of the somatic embryos of the fraxinus mandshurica, reduces the somatic embryo abnormal rate to less than 50 percent, and improves the somatic embryo development consistency to more than 80 percent and the survival rate to 64 to 81 percent.

Description

technical field

[0001] The invention relates to a method for improving the synchronization of somatic embryo development. Background technique

[0002] Fraxinus mandshurica Rupr. belongs to the genus Fraxinus mandshurica Rupr., and is one of the important and precious hard broad tree species in Northeast my country. The trunk is straight, the tree is long, the wood material is fine, and the texture is beautiful. It is widely used in military industry, construction, machinery, aviation, vehicles, room decoration, and sports equipment manufacturing. Fraxinus mandshurica seeds have the characteristics of deep dormancy. The use of traditional seeds to propagate seedlings has problems such as long seed treatment time, slow seedling formation, and long reproductive cycle. Seedlings. Somatic cell embryo development technology in plant tissue culture technology is favored by people for its advantages of large number of reproduction, fast reproduction speed, and stable genetic trai...