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Method for improving Fraxinus mandshurica somatic embryo development synchronization

A cell embryo, synchronization technology, applied in plant cells and other directions, can solve the problems of low survival rate of somatic embryo regeneration, asynchrony of somatic embryo development, and high malformation rate of somatic embryos, so as to avoid abnormal development in the later stage. , low cost, and the effect of improving the survival rate

Inactive Publication Date: 2009-08-12
NORTHEAST FORESTRY UNIVERSITY +2
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of unsynchronized somatic embryo development in the existing ash somatic embryo development technology, poor consistency of somatic embryo development, resulting in low survival rate of somatic embryo regenerated plants and the use of chemical regulation, It leads to the problem of high malformation rate of somatic embryos, and provides a method to improve the synchronization of somatic embryo development in Mandshurica mandshurica

Method used

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specific Embodiment approach 1

[0007] Specific embodiment one: the method for improving the synchronous development of Mandshurica mandshurica somatic embryos in this embodiment is realized by the following steps: 1. Inoculate the explants of Mandshurica mandshurica in the somatic embryo induction medium at a temperature of 22 to 30° C. Cultivate spherical somatic embryos under conditions of no light with a humidity of 60% to 70% until spherical somatic embryos are obtained; 2. Inoculate spherical somatic embryos in a synchronously regulated medium at a temperature of 22 to 30°C and a humidity of 60% to 70 %, every 20 to 30 days to replace the fresh synchronous control medium and culture it for 40 to 60 days under the condition of no light to obtain mature somatic embryos; 3. Strip the mature somatic embryos from the explants and place them in sterile In an empty petri dish, dry it for 10-30min under natural light conditions with a temperature of 22-30°C and a humidity of 60%-70%, and then inoculate it in th...

specific Embodiment approach 2

[0014] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, the culture is carried out under the condition of no light at a temperature of 25° C. and a humidity of 65%. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0015] Specific embodiment 3: The difference between this embodiment and specific embodiment 2 is that in step 2, the temperature is 25°C, the humidity is 65%, and the fresh synchronization regulation medium is replaced every 25 days without light for 50 days. Other steps and parameters are the same as in the second embodiment.

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Abstract

The invention discloses a method for improving the synchronization of the development of the somatic embryos of fraxinus mandshurica, which relates to a method for improving the synchronization of the development of somatic embryos. The method solves the problems of the prior technology for the development of the somatic embryos of the fraxinus mandshurica, such as asynchronous development of the somatic embryos, low survival rate of somatic embryo regeneration plants caused by low somatic embryo development consistency, and high somatic embryo abnormal rate caused by chemical regulation. The method comprises the following steps that: I) explants are inoculated in a somatic embryo inductive culture medium to be cultured to form spherical somatic embryos; II) the spherical somatic embryos are inoculated in a synchronization regulating culture medium to be cultured to form mature somatic embryos; and III) the mature somatic embryos are inoculated in a germination culture medium after being dried to be cultured to form fraxinus mandshurica plantlets, and thus the improvement in the synchronization of the development of the somatic embryos of the fraxinus mandshurica is completed. The method realizes the physical regulation of the synchronous development of the somatic embryos of the fraxinus mandshurica, reduces the somatic embryo abnormal rate to less than 50 percent, and improves the somatic embryo development consistency to more than 80 percent and the survival rate to 64 to 81 percent.

Description

technical field [0001] The invention relates to a method for improving the synchronization of somatic embryo development. Background technique [0002] Fraxinus mandshurica Rupr. belongs to the genus Fraxinus mandshurica Rupr., and is one of the important and precious hard broad tree species in Northeast my country. The trunk is straight, the tree is long, the wood material is fine, and the texture is beautiful. It is widely used in military industry, construction, machinery, aviation, vehicles, room decoration, and sports equipment manufacturing. Fraxinus mandshurica seeds have the characteristics of deep dormancy. The use of traditional seeds to propagate seedlings has problems such as long seed treatment time, slow seedling formation, and long reproductive cycle. Seedlings. Somatic cell embryo development technology in plant tissue culture technology is favored by people for its advantages of large number of reproduction, fast reproduction speed, and stable genetic trai...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 沈海龙杨玲张宇
Owner NORTHEAST FORESTRY UNIVERSITY
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