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Silkworm cocoon reelability evaluating method

An evaluation method, cocoon technology, which is applied in the evaluation field of silkworm cocoon unwinding, can solve problems such as inaccuracy, inability to quickly obtain results, complicated operation, etc., and achieve the effect of correct evaluation

Inactive Publication Date: 2009-08-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the evaluation of cocoon unwinding of silkworm silkworm is mainly based on the difficulty of swelling and solubility of cocoon layer sericin, the initial dissolution rate coefficient of cocoon layer sericin, the size of cocoon unwinding index, the value of fluorescence color rendering of fresh silkworm cocoons, and the degree of cocoon layer cocoon. Judging or detecting the quality of silkworm cocoon unwinding such as silk peeling resistance tension, these methods have problems such as slow speed, complicated operation, instability or inaccuracy
However, although the method of silk reeling detection has high accuracy, the result cannot be obtained quickly

Method used

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  • Silkworm cocoon reelability evaluating method
  • Silkworm cocoon reelability evaluating method
  • Silkworm cocoon reelability evaluating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The evaluation method flow when the incident angle is 30°: cut the silkworm cocoon 1 as the sample cocoon and pour out the silkworm chrysalis, then cut the cocoon layer into cocoon pieces 2 with a length of about 30 mm and a width of about 15 mm; randomly take 2 pieces of cocoon pieces, and the same On the cocoon layer, put it into the sample fixture of the general infrared spectrophotometer total reflection device (FTIR-8400S, SIMATSU, JAPAN), and the cocoon piece should be tightly attached to the prism and fixed in the fixture to keep the cocoon piece as flat as possible. Then, the infrared light is reflected on the surface of the cocoon sheet at an angle of 30° of incidence, and the measured 2000-500cm -1 The absorption spectrum in the spectral range, read the structural information value on the amide V on the computer display of the infrared spectrometer. Through the numerical conversion relationship table, the value of the cocoon unwinding rate is obtained, and the...

Embodiment 2

[0026] The evaluation method flow when the incident angle is 45°: except that the incident angle is 45° and the structural information value on the amide II, the rest is the same as the evaluation method flow of the embodiment 1 with an incident angle of 30°. The error range of the unwinding rate (%) is -3.17~+2.39.

[0027] When adopting the evaluation method of the outer cocoon layer at an incident angle of 45°, the dependence relationship between the silkworm cocoon unwinding rate (%) and the structural information value of the cocoon layer sericin structure at the amide II 1514cm-1 place is:

[0028] x 45 = ln [ 262 . 7034 y 45 - 1.4015 ] / 0.5116

[0029] In the formula:

[0030] the y 45 ——Relief ra...

Embodiment 3

[0034] The evaluation method flow when the incident angle is 60°: except that the incident angle is 60° and the structural information value on the amide I, the rest is the same as the evaluation method flow of the embodiment 1 with an incident angle of 30°. The error range of the unwinding rate (%) is -3.41~+2.39.

[0035] When adopting the evaluation method of the outer cocoon layer at an incident angle of 60°, the dependence relationship between the silkworm cocoon unwinding rate (%) and the structural information value of the cocoon layer sericin structure at the amide I 1612cm-1 place is:

[0036] x 60 = ln [ 409.7858 y 60 - 3.1969 ] / 0 . 5811

[0037] In the formula:

[0038] the y 60 ——The unwinding rate at an incident angle of 6...

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Abstract

The invention discloses a method for evaluating the reelability of cocoon, which comprises the following steps of: putting cocoon forks of the cocoon in a total-reflection device of an infrared spectrophotometer; projecting the cocoon forks onto an outer surface layer of the cocoon forks with a certain angle of incidence; obtaining infrared spectrum information of structure changes of sericin on the cocoon shell surface; and selecting a structure information value at any one of amide I, II, or V as a representative value of the cocoon reelability percentage. When the structure information value is big, the cocoon reelability is poor; and when the structure information value is small, the cocoon reelability is good. The method has the beneficial effects that the total-reflection mode of the infrared spectrophotometer is used for evaluating the structure information value of the secondary structure of sericin molecules on the cocoon shell surface of the cocoon, and the value of the cocoon reelability percentage is obtained by numerical transformational relation, thus being a method for evaluating the reelability of cocoon rapidly, simply, conveniently and correctly.

Description

technical field [0001] The invention relates to an evaluation technology for silkworm cocoon unwinding, in particular to an evaluation method for silkworm silkworm cocoon unwinding that can judge the quality of silkworm cocoon unwinding or the rate of unwinding. Background technique [0002] At present, the evaluation of cocoon unwinding of silkworm silkworm is mainly based on the difficulty of swelling and solubility of cocoon layer sericin, the initial dissolution rate coefficient of cocoon layer sericin, the size of cocoon unwinding index, the value of fluorescence color rendering of fresh silkworm cocoons, and the degree of cocoon layer cocoon. Judging or detecting the quality of silkworm cocoon unwinding such as silk peeling resistance tension, these methods have problems such as slow speed, complicated operation, instability or inaccuracy. And by the method of silk reeling detection, though correctness is high, can't obtain result fast. [0003] In order to overcome t...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N21/84
Inventor 朱良均张海萍闵思佳
Owner ZHEJIANG UNIV
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