Blood cell separation

A cell and fetal cell technology, applied in analytical materials, biomaterial analysis, material inspection products, etc., can solve problems such as changing the physiological characteristics of cells

Inactive Publication Date: 2009-09-02
UNIV OF THE WEST OF ENGLAND BRISTOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, existing density gradient-based methods may alter the physiological properties of cells (Hahn, S et al. Molecular Human Reproduction (1998) 4 515-521)

Method used

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  • Blood cell separation
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Experimental program
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Embodiment Construction

[0072] Heat shock protein 60 is identified as fetal cell surface specific

[0073] By comparing the proteins expressed in fetal erythroid cell membranes and adult erythrocyte membranes, HSP-60 was identified as specific to the fetal erythroid cell surface.

[0074] Specifically, a red blood cell ghost film prepared and stored at -80°C is used. After optimizing the membrane dissolution protocol, it was found that a mixture of detergents ASB-14 and CHAPS at concentrations of 0.4% and 1.2%, respectively, produced the best results. In each two-dimensional electrophoresis experiment, 25 μg of dissolved membrane was used. Focusing is achieved by using a fixed pH gradient and is enhanced by adding ampholytes (mixtures of amphiphiles with various pI values) to the sample loading buffer. The protein is loaded onto the anode, and a current is applied to the gel strip. When the proteins move to the cathode, they are fixed at a position where their net electricity is zero, which is their isoe...

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Abstract

There is provided a method of isolating foetal cells from an isolated sample of maternal blood, the method comprising identifying cells having a different expression pattern of at least one foetal marker compared to the expression pattern of the marker in an equivalent maternal cell and selecting the identified cells, characterised in that the foetal marker is selected from: HSP-60, a monoamine oxidase, glutamine synthase, Ara-70, Ara-54, FLJ20202, DCN-I protein, RAB5A, HSP-7C, EFlAl, GRP78, MYL4, DnaJ homolog subfamily B member 14, Vinculin, Desmoplakin, AMMECRl -like protein, Extracellular matrix protein 2 precursor protein, uncharacterised protein Cxorf57, Peroxiredoxin 1, Peroxiredoxin 2. There is also provided a method of cultivating foetal cells and a foetal cell isolation kit.

Description

Technical field [0001] The present invention relates to the field of prenatal diagnosis, in particular to the separation of fetal cells from maternal peripheral blood. Specifically, the present invention relates to a method for separating fetal cells from maternal peripheral blood and a device for separating fetal blood cells from maternal peripheral blood. Background technique [0002] Existing methods of prenatal diagnosis of diseases involve invasive techniques. For example, such techniques include amniocentesis, chorionic villus sampling, and umbilical puncture. Currently, millions of such analyses are performed every year using materials sampled through invasiveness to detect chromosomal abnormalities (such as Down syndrome) and other genetic disorders. In the United States alone, approximately 200,000 invasive prenatal testing procedures are performed each year, such as amniocentesis and chorionic villus sampling. Such tests are usually performed on women over 35 and those ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/6893G01N2800/38
Inventor N·D·埃文特Z·E·普卢默D·J·黑德
Owner UNIV OF THE WEST OF ENGLAND BRISTOL
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