Method for separating genistein monomer from daidzein monomer

A separation method, the technology of genistein, applied in the field of separation of genistein and daidzein monomer, can solve the problems that are difficult to be extended to industrial implementation, low separation efficiency, high separation cost, etc., and achieve pollution-free purity, simple operation, The effect of efficient selective separation

Inactive Publication Date: 2011-09-14
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing separation method is not only complicated to operate, but also has a high cost of separation. The key is to obtain a high-purity monomer through multiple separation operations before it is possible to obtain a trace amount of sample. The separation efficiency is low, and it is difficult to be extended to industrial implementation.

Method used

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  • Method for separating genistein monomer from daidzein monomer
  • Method for separating genistein monomer from daidzein monomer
  • Method for separating genistein monomer from daidzein monomer

Examples

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Embodiment

[0026] a) Preparation of gold nanochannel membrane

[0027] Immerse a 30nm porous polycarbonate (PC) membrane in anhydrous methanol and ultrasonically vibrate for 5 minutes to wash away the impurities adsorbed on the base membrane and activate the membrane; the cleaned PC membrane is sequentially exposed to SnCl with a concentration of 25mM 2 Soak in the solution for 45 minutes → take it out and wash it twice with water, then the concentration of Ag(NH 3 ) 2 + Soak in the solution for 15 minutes → take out and wash with water for 4 times, then soak in methanol for 5 minutes → take out Electroless gold plating at 1°C for 6 hours in a gold sodium sulfite deposition solution (pH = 10) with a concentration of 7.9mM → take out with water After washing 4 times, use 25% HNO 3 After soaking in aqueous solution for 12 hours, a gold nanochannel membrane with a diameter of 10 nm is obtained; figure 1 It is a scanning electron microscope (SEM) image of the surface of the prepared gold...

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Abstract

The invention discloses a method for separating a genistein monomer from a daidzein monomer. The method comprises a preparation process of a gold nanotubule membrane, a modification process of the gold nanotubule membrane, a preparation process of an aluminum chelate and a membrane separation process. The method helps realize high-efficiency selective separation of the genistein monomer and the daidzein monomer by utilizing mechanism that the gold nanotubule membrane is modified with p-aminothiophenol so that the membrane is positively charged at a lower pH value, and plays electrostatic repulsion on positively charged genistein and aluminum chelate without repulsion on daidzein which can not form a chelate with the aluminum. The method has the advantages of simple operation, low energy consumption and high efficiency of separation, no pollution, high purity of the monomers separated, industrialized implementation and the like. The method is of great theoretical significance for further research on pharmacological action, mechanism, popularization and application of genistein and daidzein.

Description

technical field [0001] The invention relates to a method for separating genistein and daidzein monomers, in particular to a method for separating genistein and daidzein monomers in soybean isoflavones, and belongs to the technical field of separation and purification of natural medicines. Background technique [0002] Studies have shown that genistein (alias genistein, English name Genistein) has estrogen and anti-estrogen properties, can inhibit colon cancer, breast cancer, skin cancer, prostate cancer, etc. in vivo, and has inhibitory effects on various tumor cell lines. Function; has antioxidant effect, can prevent postmenopausal osteoporosis; has the effects of inducing programmed cell death, improving anticancer drug efficacy, and inhibiting angiogenesis. It is a potential cancer chemopreventive agent, and its anticancer effect And the mechanism has broad application prospects. Daidzein (alias daidzein, English name Daidzein) has estrogen-like effect and obvious anti-h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07D311/40B01D61/18C23C18/44A61P35/00A61P39/06
Inventor 黄杉生彭斌岳增连赵国庆
Owner SHANGHAI NORMAL UNIVERSITY
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